| http://purl.uniprot.org/citations/12107078 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12107078 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12107078 | http://www.w3.org/2000/01/rdf-schema#comment | "alpha1,3-Fucosyltransferases (Fuc-Ts) convert N-acetyllactosamine (LN, Galbeta1-4GlcNAc) to Galbeta1-4(Fucalpha1-3)GlcNAc, the Lewis x (CD15, SSEA-1) epitope, which is involved in various recognition phenomena. We describe details of the acceptor specificity of alpha1,3-fucosyltransferase IX (Fuc-TIX). The unconjugated N- and O-glycan analogs LNbeta1-2Man, LNbeta1-6Manalpha1-OMe, LNbeta1-2Manalpha1-3(LNbeta1-2Manalpha1-6)Manbeta1-4GlcNAc, and Galbeta1-3(LNbeta1-6)GalNAc reacted well in vitro with Fuc-TIX present in lysates of appropriately transfected Namalwa cells. Fuc-TIX reacted well with the reducing end LN of GlcNAcbeta1-3'LN (underscored site reacted) and GlcNAcbeta1-3'LNbeta1-3'LN (both LNs reacted), but very poorly with the reducing end LN of LNbeta1-3'LN. However, Fuc-TIX reacted significantly better with the non-reducing end LN as compared to the other LN units in the glycans LNbeta1-3'LN and LNbeta1-3'LNbeta1-3'LNbeta1-3'LN, confirming our previous data on LNbeta1-3'LNbeta1-OR. In contrast, the sialylated glycan Neu5Acalpha2-3'LNbeta1-3'LNbeta1-3'LNbeta1-3'LN was fucosylated preferentially at the two most reducing end LN units. We conclude that Fuc-TIX is a versatile alpha1,3-Fuc-T, that (1) generates distal Lewis x epitopes from many different acceptors, (2) possesses inherent ability for the biosynthesis of internal Lewis x epitopes on growing polylactosamine backbones, and (3) fucosylates the remote internal LN units of alpha2,3-sialylated i-type polylactosamines."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/12.6.361"xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/12.6.361"xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/author | "Nishihara S."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/author | "Nishihara S."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/author | "Narimatsu H."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/author | "Narimatsu H."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/author | "Renkonen R."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/author | "Renkonen R."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/author | "Renkonen O."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/author | "Renkonen O."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/author | "Toivonen S."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/author | "Toivonen S."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/pages | "361-368"xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/pages | "361-368"xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/title | "Fuc-TIX: a versatile alpha1,3-fucosyltransferase with a distinct acceptor- and site-specificity profile."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/title | "Fuc-TIX: a versatile alpha1,3-fucosyltransferase with a distinct acceptor- and site-specificity profile."xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/12107078 | http://purl.uniprot.org/core/volume | "12"xsd:string |