| http://purl.uniprot.org/citations/12111824 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12111824 | http://www.w3.org/2000/01/rdf-schema#comment | "The properties of a transport system specific for gamma-aminobutyric acid (GABA) expressed in human U373 MG astrocytoma cells were examined. The uptake of [(3)H]GABA was dependent on both extracellular Na(+) and Cl(-) ions and was inhibited by (+/-)-nipecotic acid, guvacine, and beta-alanine, with a pharmacological profile corresponding to that reported for the human homologue of the GABA/betaine transporter (BGT-1). Accordingly, [(3)H]GABA uptake was also inhibited by betaine, and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of total RNA from U373 MG cells with specific BGT-1 primers resulted in the amplification of a 440 bp fragment that was further characterized by restriction analysis and sequencing. In addition, Western blot analysis with anti-BGT-1 antiserum revealed the presence of a characteristic 60 kDa band. The primary structure of the human BGT-1 protein predicts two putative phosphorylation sites for the Ca(2+)/diacylglicerol-dependent protein kinase (PKC), and treatment of U373 MG cells with the PKC activator phorbol 12-myristate-13-acetate (TPA) led to a concentration- and time-dependent decrease in [(3)H]GABA uptake. The maximal effect was detected at 2 hr of incubation, to disappear after 4 hr. TPA-induced reduction in [(3)H]GABA uptake was reversed by preincubation with staurosporine. Taken together, these results indicate that U373 MG cells express a GABA transporter of the BGT-1 subtype whose function is regulated by phosphorylation events through PKC."xsd:string |
| http://purl.uniprot.org/citations/12111824 | http://purl.org/dc/terms/identifier | "doi:10.1002/jnr.10258"xsd:string |
| http://purl.uniprot.org/citations/12111824 | http://purl.uniprot.org/core/author | "Ortega A."xsd:string |
| http://purl.uniprot.org/citations/12111824 | http://purl.uniprot.org/core/author | "Arias-Montano J.A."xsd:string |
| http://purl.uniprot.org/citations/12111824 | http://purl.uniprot.org/core/author | "Ruiz-Tachiquin M.E."xsd:string |
| http://purl.uniprot.org/citations/12111824 | http://purl.uniprot.org/core/author | "Sanchez-Lemus E."xsd:string |
| http://purl.uniprot.org/citations/12111824 | http://purl.uniprot.org/core/author | "Soria-Jasso L.E."xsd:string |
| http://purl.uniprot.org/citations/12111824 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12111824 | http://purl.uniprot.org/core/name | "J Neurosci Res"xsd:string |
| http://purl.uniprot.org/citations/12111824 | http://purl.uniprot.org/core/pages | "125-132"xsd:string |
| http://purl.uniprot.org/citations/12111824 | http://purl.uniprot.org/core/title | "Gamma-aminobutyric acid transporter (BGT-1) expressed in human astrocytoma U373 MG cells: pharmacological and molecular characterization and phorbol ester-induced inhibition."xsd:string |
| http://purl.uniprot.org/citations/12111824 | http://purl.uniprot.org/core/volume | "69"xsd:string |
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