| http://purl.uniprot.org/citations/12123444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12123444 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12123444 | http://www.w3.org/2000/01/rdf-schema#comment | "Escherichia coli O157:H7 causes diarrhoea, haemorrhagic colitis, and the haemolytic uraemic syndrome. We have identified a protein of previously unknown function encoded on the pO157 virulence plasmid of E. coli O157:H7, which is the first described protease that specifically cleaves C1 esterase inhibitor (C1-INH), a member of the serine protease inhibitor family. The protein, named StcE for secreted protease of C1 esterase inhibitor from EHEC (formerly Tagn), cleaves C1-INH to produce (unique) approximately 60-65 kDa fragments. StcE does not digest other serine protease inhibitors, extracellular matrix proteins or universal protease targets. We also observed that StcE causes the aggregation of cultured human T cells but not macrophage-like cells or B cells. Substitution of aspartic acid for glutamic acid at StcE position 435 within the consensus metalloprotease active site ablates its abilities to digest C1-INH and to aggregate T cells. StcE is secreted by the etp type II secretion pathway encoded on pO157, and extracellular StcE levels are positively regulated by the LEE-encoded regulator, Ler. StcE antigen and activity were detected in the faeces of a child with an E. coli O157:H7 infection, demonstrating the expression of StcE during human disease. Cleavage of C1-INH by StcE could plausibly cause localized pro-inflammatory and coagulation responses resulting in tissue damage, intestinal oedema and thrombotic abnormalities."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1365-2958.2002.02997.x"xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1365-2958.2002.02997.x"xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Torres A.G."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Torres A.G."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Welch R.A."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Welch R.A."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Kaper J.B."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Kaper J.B."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Tarr P.I."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Tarr P.I."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Grys T.E."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Grys T.E."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Lathem W.W."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Lathem W.W."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Witowski S.E."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/author | "Witowski S.E."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/name | "Mol. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/pages | "277-288"xsd:string |
| http://purl.uniprot.org/citations/12123444 | http://purl.uniprot.org/core/pages | "277-288"xsd:string |