Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/12123777http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12123777http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12123777http://www.w3.org/2000/01/rdf-schema#comment"

Background

Dendroaspis natriuretic peptide (DNP) is the newest member of the natriuretic peptide family and is a circulating peptide in humans. The effects of DNP on the human vasculature are unknown. Since other natriuretic peptides are known to cause vasorelaxation, we determined the response to DNP on human blood vessels in vitro. We also investigated the mechanism of DNP mediated vasorelaxation.

Methods

Rings of human internal mammary artery and saphenous vein were suspended in an organ bath. The response to cumulative concentrations of DNP was obtained. Inhibiting agents were used to determine the mechanism of this vasorelaxation.

Results

DNP caused dose-dependent relaxation, with a greater effect on the internal mammary arteries (relaxation from 10(-7) mol/l DNP: 80.6+/-4.1%) than the saphenous veins (33.4+/-4.1%). At 10(-7) mol/l, DNP resulted in less arterial relaxation compared with atrial and C-type natriuretic peptides and similar relaxation to brain natriuretic peptide. In veins, DNP caused the greatest relaxation of the natriuretic peptides. DNP increased tissue cyclic guanosine monophosphate (cGMP) determined by radioimmunoassay by over 7-fold. Barium chloride and indomethacin attenuated DNP mediated vasorelaxation. However, glibenclamide, charydotoxin, apamin, tetraethyl-ammonium chloride and diisothiocyanato-stilbene-2,2'-disulfonic acid did not. DNP mediated vasorelaxation was mildly attenuated with removal of the endothelium. DNP immunoreactivity was identified in both arteries and veins.

Conclusions

The current study demonstrates that DNP is an endogenous human natriuretic peptide that relaxes human arteries more than veins. Furthermore, DNP mediated vasorelaxation involves the inward rectifying potassium channels, prostaglandins, and cGMP. This newest member of the natriuretic peptide family may have an important physiologic role in the human vasculature."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.org/dc/terms/identifier"doi:10.1016/s0008-6363(02)00402-9"xsd:string
http://purl.uniprot.org/citations/12123777http://purl.org/dc/terms/identifier"doi:10.1016/s0008-6363(02)00402-9"xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/author"Wilson S.H."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/author"Wilson S.H."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/author"Burnett J.C."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/author"Burnett J.C."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/author"Best P.J."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/author"Best P.J."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/author"Holmes D.R. Jr."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/author"Holmes D.R. Jr."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/author"Lerman A."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/author"Lerman A."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/name"Cardiovasc. Res."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/name"Cardiovasc. Res."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/pages"375-384"xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/pages"375-384"xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/title"Dendroaspis natriuretic peptide relaxes isolated human arteries and veins."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/title"Dendroaspis natriuretic peptide relaxes isolated human arteries and veins."xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/volume"55"xsd:string
http://purl.uniprot.org/citations/12123777http://purl.uniprot.org/core/volume"55"xsd:string
▶