| http://purl.uniprot.org/citations/12138164 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12138164 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12138164 | http://www.w3.org/2000/01/rdf-schema#comment | "Heparan sulfate 3-O-sulfotransferase transfers sulfate to the 3-OH position of a glucosamine residue of heparan sulfate (HS) to form 3-O-sulfated HS. The 3-O-sulfated glucosamine residue contributes to two important biological functions of HS: binding to antithrombin and thereby carrying anticoagulant activity, and binding to herpes simplex viral envelope glycoprotein D to serve as an entry receptor for herpes simplex virus 1. A total of five HS 3-O-sulfotransferase isoforms were reported previously. Here we report the isolation and characterization of a novel HS 3-O-sulfotransferase isoform, designated as HS 3-O-sulfotransferase isoform 5 (3-OST-5). 3-OST-5 cDNA was isolated from a human placenta cDNA library and expressed in COS-7 cells. The disaccharide analysis of 3-OST-5-modified HS revealed that 3-OST-5 generated at least three 3-O-sulfated disaccharides as follows: IdoUA2S-AnMan3S, GlcUA-AnMan3S6S, and IdoUA2S-AnMan3S6S. Transfection of the plasmid expressing 3-OST-5 rendered wild type Chinese hamster ovary cells susceptible to the infection by herpes simplex virus 1, suggesting that 3-OST-5-modified HS serves as an entry receptor for herpes simplex virus 1. In addition, 3-OST-5-modified HS bound to herpes simplex viral envelope protein glycoprotein D. Furthermore, we found that 3-OST-5-modified HS also bound to antithrombin, suggesting that 3-OST-5 also produces anticoagulant HS. In summary, our results indicate that a new member of 3-OST family generates both anticoagulant HS and an entry receptor for herpes simplex virus 1. These results provide a new insight regarding the mechanism for the biosynthesis of biologically active HS."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m204209200"xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m204209200"xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Chen J."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Chen J."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Shukla D."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Shukla D."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Xia G."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Xia G."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Tiwari V."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Tiwari V."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Li J.-P."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Li J.-P."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Ju W."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Ju W."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Malmstroem A."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/author | "Malmstroem A."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12138164 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |