| http://purl.uniprot.org/citations/12149401 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12149401 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12149401 | http://www.w3.org/2000/01/rdf-schema#comment | "Thioredoxins are proteins that participate in different cellular processes via redox-mediated reactions. For humans, we have recently described two novel members of this family that display a male germ cell specific expression pattern, named spermatid specific thioredoxin (Sptrx-1 and Sptrx-2 respectively). We report here the cloning and characterization of the mouse Sptrx-1 gene and protein, which are similar to those described for the human orthologue. The mouse Sptrx-1 open reading frame encodes for a protein of 462 aa composed of an N-terminal repetitive domain of a 15 residue motif followed by a C-terminal domain typical of thioredoxins. The mouse Sptrx-1 gene sequence is interrupted by only one intron of 525 bp located in the 5'-UTR, and using fluorescence in-situ hybridization we have mapped its chromosomal location to 17E1.2-1.3. Northern blot analysis identified the testis as the only tissue expressing mouse Sptrx-1 mRNA, and by in-situ hybridization we found a strong labelling in the testicular seminiferous tubules, mostly in the round spermatids. Affinity purified antibodies against human Sptrx-1 crossreacted well with the mouse protein confirming its expression in seminiferous tubules at the later stages of spermiogenesis. Recombinant mouse Sptrx-1 displayed protein disulphide reducing activity in an enzymatic assay coupled to NADPH and thioredoxin reductase. The availability of the mouse Sptrx-1 gene sequence is the first step towards the generation of knock-out mice, whose characterization will provide significant information regarding the in-vivo function of Sptrx-1 and its possible implication in several sperm anomalies."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.org/dc/terms/identifier | "doi:10.1093/molehr/8.8.710"xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.org/dc/terms/identifier | "doi:10.1093/molehr/8.8.710"xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Gustafsson J.-A."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Gustafsson J.-A."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Jimenez A."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Jimenez A."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Pelto-Huikko M."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Pelto-Huikko M."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Oko R."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Oko R."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Miranda-Vizuete A."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Miranda-Vizuete A."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Spyrou G."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/author | "Spyrou G."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/name | "Mol. Hum. Reprod."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/name | "Mol. Hum. Reprod."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/pages | "710-718"xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/pages | "710-718"xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/title | "Cloning, expression and characterization of mouse spermatid specific thioredoxin-1 gene and protein."xsd:string |
| http://purl.uniprot.org/citations/12149401 | http://purl.uniprot.org/core/title | "Cloning, expression and characterization of mouse spermatid specific thioredoxin-1 gene and protein."xsd:string |