| http://purl.uniprot.org/citations/12154172 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12154172 | http://www.w3.org/2000/01/rdf-schema#comment | "Different subtypes of voltage-dependent Ca(2+) currents in native neurones are essential in coupling action potential firing to Ca(2+) influx. For most of these currents, the underlying Ca(2+) channel subunits have been identified on the basis of pharmacological and biophysical similarities. In contrast, the molecular basis of R-type Ca(2+) currents remains controversial. We have therefore examined the contribution of the Ca(V)2.3 (alpha(1E)) subunits to R-type currents in different types of central neurones using wild-type mice and mice in which the Ca(V)2.3 subunit gene was deleted. In hippocampal CA1 pyramidal cells and dentate granule neurones, as well as neocortical neurones of wild-type mice, Ca(2+) current components resistant to the combined application of omega-conotoxin GVIA and MVIIC, omega-agatoxin IVa and nifedipine (I(Ca,R)) were detected that were composed of a large R-type and a smaller T-type component. In Ca(V)2.3-deficient mice, I(Ca,R) was considerably reduced in CA1 neurones (79 %) and cortical neurones (87 %), with less reduction occurring in dentate granule neurones (47 %). Analysis of tail currents revealed that the reduction of I(Ca,R) is due to a selective reduction of the rapidly deactivating R-type current component in CA1 and cortical neurones. In all cell types, I(Ca,R) was highly sensitive to Ni(2+) (100 microM: 71-86 % block). A selective antagonist of cloned Ca(V)2.3 channels, the spider toxin SNX-482, partially inhibited I(Ca,R) at concentrations up to 300 nM in dentate granule cells and cortical neurones (50 and 57 % block, EC(50) 30 and 47 nM, respectively). I(Ca,R) in CA1 neurones was significantly less sensitive to SNX-482 (27 % block, 300 nM SNX-482). Taken together, our results show clearly that Ca(V)2.3 subunits underlie a significant fraction of I(Ca,R) in different types of central neurones. They also indicate that Ca(V)2.3 subunits may give rise to Ca(2+) currents with differing pharmacological properties in native neurones."xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.org/dc/terms/identifier | "doi:10.1113/jphysiol.2002.020677"xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/author | "Schneider T."xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/author | "Pereverzev A."xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/author | "Smyth N."xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/author | "Beck H."xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/author | "Gissel C."xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/author | "Sochivko D."xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/name | "J Physiol"xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/pages | "699-710"xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/title | "The Ca(V)2.3 Ca(2+) channel subunit contributes to R-type Ca(2+) currents in murine hippocampal and neocortical neurones."xsd:string |
| http://purl.uniprot.org/citations/12154172 | http://purl.uniprot.org/core/volume | "542"xsd:string |
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