| http://purl.uniprot.org/citations/12163372 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12163372 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12163372 | http://www.w3.org/2000/01/rdf-schema#comment | "Conversion of fibroblasts into myofibroblasts as mediated by transforming growth factor-beta1 (TGF-beta1) is the most prominent stromal reaction to a number of epithelial lesions including breast cancer. To identify genes which are regulated during this process, the mRNA profiles from primary breast fibroblasts treated with or without TGF-beta1 were analyzed by differential display. Ninety-five differentially expressed transcripts were PCR-cloned and sequenced, and 28 clones were selected for verification in a hybridization array. By use of gene-specific sequence tags, nine differentially expressed genes were identified. One of the clones, identified as CLIC4, a member of the CLIC family of chloride channels, was up-regulated more than 16 times in myofibroblasts and was therefore chosen for further analysis. Using RT-PCR, comparison with CLIC1, CLIC2, CLIC3, and CLIC5 demonstrated that CLIC4 was unique by being up-regulated by TGF-beta1 in myofibroblasts. Immunohistochemistry showed a hitherto unknown, distinctive pattern of CLIC4 expression in breast stroma. Whereas normal breast fibroblasts were devoid of CLIC4 protein expression, myofibroblasts of breast carcinomas were strongly CLIC4-positive. The functional significance of CLIC4 was analyzed in MEF/3T3 fibroblasts by conditional expression using the tetracycline-repressive gene regulation system. In a migration assay, we found that CLIC4 inhibited cell motility by 27%. These results suggest that CLIC4 is differentially regulated in fibroblasts and that its expression contributes to a collective stationary myofibroblast phenotype."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0002-9440(10)64203-4"xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0002-9440(10)64203-4"xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/author | "Edwards J.C."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/author | "Edwards J.C."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/author | "Petersen O.W."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/author | "Petersen O.W."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/author | "Ronnov-Jessen L."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/author | "Ronnov-Jessen L."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/author | "Villadsen R."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/author | "Villadsen R."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/name | "Am. J. Pathol."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/name | "Am. J. Pathol."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/pages | "471-480"xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/pages | "471-480"xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/title | "Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/title | "Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts."xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/volume | "161"xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://purl.uniprot.org/core/volume | "161"xsd:string |
| http://purl.uniprot.org/citations/12163372 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12163372 |
| http://purl.uniprot.org/citations/12163372 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12163372 |