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http://purl.uniprot.org/citations/12163479http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12163479http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12163479http://www.w3.org/2000/01/rdf-schema#comment"AKAP350 can scaffold a number of protein kinases and phosphatases at the centrosome and the Golgi apparatus. We performed a yeast two-hybrid screen of a rabbit parietal cell library with a 3.2-kb segment of AKAP350 (nucleotides 3611-6813). This screen yielded a full-length clone of rabbit chloride intracellular channel 1 (CLIC1). CLIC1 belongs to a family of proteins, all of which contain a high degree of homology in their carboxyl termini. All CLIC family members were able to bind a 133-amino acid domain within AKAP350 through the last 120 amino acids in the conserved CLIC carboxyl termini. Antibodies developed against a bovine CLIC, p64, immunoprecipitated AKAP350 from HCA-7 colonic adenocarcinoma cell extracts. Antibodies against CLIC proteins recognized at least five CLIC species including a novel 46-kDa CLIC protein. We isolated the human homologue of bovine p64, CLIC5B, from HCA-7 cell cDNA. A splice variant of CLIC5, the predicted molecular mass of CLIC5B corresponds to the molecular mass of the 46-kDa CLIC immunoreactive protein in HCA-7 cells. Antibodies against CLIC5B colocalized with AKAP350 at the Golgi apparatus with minor staining of the centrosomes. AKAP350 and CLIC5B association with Golgi elements was lost following brefeldin A treatment. Furthermore, GFP-CLIC5B-(178-410) targeted to the Golgi apparatus in HCA-7 cells. The results suggest that AKAP350 associates with CLIC proteins and specifically that CLIC5B interacts with AKAP350 at the Golgi apparatus in HCA-7 cells."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m112277200"xsd:string
http://purl.uniprot.org/citations/12163479http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m112277200"xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Berryman M."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Berryman M."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Edwards J.C."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Edwards J.C."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Goldenring J.R."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Goldenring J.R."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Larocca M.C."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Larocca M.C."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Navarre J."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Navarre J."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Shanks R.A."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Shanks R.A."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Urushidani T."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/author"Urushidani T."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/pages"40973-40980"xsd:string
http://purl.uniprot.org/citations/12163479http://purl.uniprot.org/core/pages"40973-40980"xsd:string