| http://purl.uniprot.org/citations/12169662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12169662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12169662 | http://www.w3.org/2000/01/rdf-schema#comment | "The covalent attachment of ubiquitin is an important determinant for selective protein degradation by the 26S proteasome in plants and animals. The specificity of ubiquitination is often controlled by ubiquitin-protein ligases (or E3s), which facilitate the transfer of ubiquitin to appropriate targets. One ligase type, the SCF E3s are composed of four proteins, cullin1/Cdc53, Rbx1/Roc1/Hrt1, Skp1, and an F-box protein. The F-box protein, which identifies the targets, binds to the Skp1 component of the complex through a degenerate N-terminal approximately 60-aa motif called the F-box. Using published F-boxes as queries, we have identified 694 potential F-box genes in Arabidopsis, making this gene superfamily one of the largest currently known in plants. Most of the encoded proteins contain interaction domains C-terminal to the F-box that presumably participate in substrate recognition. The F-box proteins can be classified via a phylogenetic approach into five major families, which can be further organized into multiple subfamilies. Sequence diversity within the subfamilies suggests that many F-box proteins have distinct functions and/or substrates. Representatives of all of the major families interact in yeast two-hybrid experiments with members of the Arabidopsis Skp family supporting their classification as F-box proteins. For some, a limited preference for Skps was observed, suggesting that a hierarchical organization of SCF complexes exists defined by distinct Skp/F-box protein pairs. Collectively, the data shows that Arabidopsis has exploited the SCF complex and the ubiquitin/26S proteasome pathway as a major route for cellular regulation and that a diverse array of SCF targets is likely present in plants."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.162339999"xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.162339999"xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/author | "Vierstra R.D."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/author | "Vierstra R.D."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/author | "Gagne J.M."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/author | "Gagne J.M."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/author | "Shiu S.-H."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/author | "Shiu S.-H."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/author | "Downes B.P."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/author | "Downes B.P."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/author | "Durski A.M."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/author | "Durski A.M."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/pages | "11519-11524"xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/pages | "11519-11524"xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/title | "The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/title | "The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis."xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/volume | "99"xsd:string |
| http://purl.uniprot.org/citations/12169662 | http://purl.uniprot.org/core/volume | "99"xsd:string |