| http://purl.uniprot.org/citations/12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12176364 | http://www.w3.org/2000/01/rdf-schema#comment | "A critical event in T cell receptor (TCR)-mediated signaling is the recruitment of hematopoietic-specific adaptor proteins that collect and transmit signals downstream of the TCR. Gads, a member of the Grb2 family of SH2 and SH3 domain-containing adaptors, mediates the formation of a complex between LAT and SLP-76 that is essential for signal propagation from the TCR. Here we examine the binding specificity of the Gads and Grb2 SH3 domains using peptide arrays and find that a nonproline-based R-X-X-K motif found in SLP-76 binds to the Gads carboxy-terminal SH3 domain with high affinity (K(D) = 240 +/-45 nM). The Grb2 C-terminal SH3 domain also binds this motif, but with a 40-fold lower affinity than Gads. Single point mutations in either the relevant R (237) or K (240) completely abrogated SLP-76 association with Gads in vivo and impaired SLP-76 function. A chimeric Grb2 protein, possessing the C-terminal SH3 domain of Gads, was able to partially substitute for Gads in signaling downstream of the T cell receptor. These results provide a molecular explanation for the specific role of Gads in T cell receptor signaling, and identify a discrete subclass of SH3 domains whose binding is dependent on a core R-X-X-K motif."xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0960-9822(02)01038-2"xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://purl.uniprot.org/core/author | "Pawson T."xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://purl.uniprot.org/core/author | "McGlade C.J."xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://purl.uniprot.org/core/author | "Nash P."xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://purl.uniprot.org/core/author | "Berry D.M."xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://purl.uniprot.org/core/author | "Liu S.K."xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12176364 | http://purl.uniprot.org/core/name | "Curr Biol"xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://purl.uniprot.org/core/pages | "1336-1341"xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://purl.uniprot.org/core/title | "A high-affinity Arg-X-X-Lys SH3 binding motif confers specificity for the interaction between Gads and SLP-76 in T cell signaling."xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/12176364 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12176364 |
| http://purl.uniprot.org/citations/12176364 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12176364 |
| http://purl.uniprot.org/uniprot/#_D3YWR2-mappedCitation-12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12176364 |
| http://purl.uniprot.org/uniprot/#_A0A2R8VH91-mappedCitation-12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12176364 |
| http://purl.uniprot.org/uniprot/#_A0A2R8VHI5-mappedCitation-12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12176364 |
| http://purl.uniprot.org/uniprot/#_A0A2I3BPY6-mappedCitation-12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12176364 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GS41-mappedCitation-12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12176364 |
| http://purl.uniprot.org/uniprot/#_A0A2I3BRH1-mappedCitation-12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12176364 |
| http://purl.uniprot.org/uniprot/#_B2RUR0-mappedCitation-12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12176364 |
| http://purl.uniprot.org/uniprot/#_B1AT92-mappedCitation-12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12176364 |
| http://purl.uniprot.org/uniprot/#_B1AT95-mappedCitation-12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12176364 |
| http://purl.uniprot.org/uniprot/#_B1ATD1-mappedCitation-12176364 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12176364 |