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http://purl.uniprot.org/citations/12177502http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12177502http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12177502http://www.w3.org/2000/01/rdf-schema#comment"Rice (Oryza sativa) anther development is easily damaged by moderately low temperatures above 12 degrees C. Subtractive screening of cDNA that accumulated in 12 degrees C-treated anthers identified a cDNA clone, OsMEK1, encoding a protein with features characteristic of a mitogen-activated protein (MAP) kinase kinase. The putative OsMEK1 protein shows 92% identity to the maize (Zea mays) MEK homolog, ZmMEK1. OsMEK1 transcript levels were induced in rice anthers by 12 degrees C treatment for 48 h. Similar OsMEK1 induction was observed in shoots and roots of seedlings that were treated at 12 degrees C for up to 24 h. It is interesting that no induction of OsMEK1 transcripts was observed in 4 degrees C-treated seedlings. In contrast, rice lip19, encoding a bZIP protein possibly involved in low temperature signal transduction, was not induced by 12 degrees C treatment but was induced by 4 degrees C treatment. Among the three MAP kinase homologs cloned, only OsMAP1 displayed similar 12 degrees C-specific induction pattern as OsMEK1. A yeast two-hybrid system revealed that OsMEK1 interacts with OsMAP1, but not with OsMAP2 and OsMAP3, suggesting that OsMEK1 and OsMAP1 probably function in the same signaling pathway. An in-gel assay of protein kinase activity revealed that a protein kinase (approximately 43 kD), which preferentially uses myelin basic protein as a substrate, was activated by 12 degrees C treatment but not by 4 degrees C treatment. Taken together, these results lead us to conclude that at least two signaling pathways for low temperature stress exist in rice, and that a MAP kinase pathway with OsMEK1 and OsMAP1 components is possibly involved in the signaling for the higher range low-temperature stress."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.org/dc/terms/identifier"doi:10.1104/pp.006072"xsd:string
http://purl.uniprot.org/citations/12177502http://purl.org/dc/terms/identifier"doi:10.1104/pp.006072"xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/author"Oono K."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/author"Oono K."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/author"Imai R."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/author"Imai R."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/author"Wen J.-Q."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/author"Wen J.-Q."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/name"Plant Physiol."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/name"Plant Physiol."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/pages"1880-1891"xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/pages"1880-1891"xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/title"Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/title"Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice."xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/volume"129"xsd:string
http://purl.uniprot.org/citations/12177502http://purl.uniprot.org/core/volume"129"xsd:string
http://purl.uniprot.org/citations/12177502http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/12177502
http://purl.uniprot.org/citations/12177502http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/12177502
http://purl.uniprot.org/citations/12177502http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/12177502
http://purl.uniprot.org/citations/12177502http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/12177502