| http://purl.uniprot.org/citations/12186975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12186975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12186975 | http://www.w3.org/2000/01/rdf-schema#comment | "Spt-Ada-Gcn5 acetyltransferase (SAGA) is a previously described histone acetyltransferase/transcriptional coactivator complex in yeast. At promoters of certain genes (HIS3 and TRP3), SAGA has an inhibitory function involving a nonproductive TATA-binding protein interaction mediated by the Spt3 and Spt8 subunits. Related to this, Spt8-less SAGA is a major form of the complex under activating conditions for these genes. In the present study, we purify this activation-specific complex, called SALSA (SAGA altered, Spt8 absent). Besides lacking Spt8, SALSA contains Spt7 subunit that is truncated. Examining the role of this subunit, we find that C-terminally truncated SPT7 resulted in derepressed HIS3 transcription. Furthermore, when grown in rich media (repressing conditions), wild-type cells yielded predominantly SAGA, but Spt7 C-terminal truncations resulted primarily in a form of complex similar to SALSA. Thus, SALSA-like structure and activating function can be partially recapitulated in yeast by truncating the C terminus of Spt7. Overall, these results lead to a model that for a subset of promoters SAGA is inhibitory through Spt3, Spt8, and an Spt8-interacting subdomain of Spt7, whereas SALSA is a form of complex for positive transcriptional regulation. These data clarify a mechanism by which a transcriptional regulatory complex can switch between positive and negative modulation."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.182021199"xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.182021199"xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/author | "Berger S.L."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/author | "Berger S.L."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/author | "Belotserkovskaya R."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/author | "Belotserkovskaya R."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/author | "Sterner D.E."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/author | "Sterner D.E."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/pages | "11622-11627"xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/pages | "11622-11627"xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/title | "SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/title | "SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription."xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/volume | "99"xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://purl.uniprot.org/core/volume | "99"xsd:string |
| http://purl.uniprot.org/citations/12186975 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12186975 |
| http://purl.uniprot.org/citations/12186975 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12186975 |
| http://purl.uniprot.org/citations/12186975 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12186975 |
| http://purl.uniprot.org/citations/12186975 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12186975 |