| http://purl.uniprot.org/citations/12192410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12192410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12192410 | http://www.w3.org/2000/01/rdf-schema#comment | "Large-conductance (BK type) Ca(2+)-dependent K(+) channels are essential for modulating muscle contraction and neuronal activities such as synaptic transmission and hearing. BK channels are activated by membrane depolarization and intracellular Ca(2+) and Mg(2+) (refs 6-10). The energy provided by voltage, Ca(2+) and Mg(2+) binding are additive in activating the channel, suggesting that these signals open the activation gate through independent pathways. Here we report a molecular investigation of a Mg(2+)-dependent activation mechanism. Using a combined site-directed mutagenesis and structural analysis, we demonstrate that a structurally new Mg(2+)-binding site in the RCK/Rossman fold domain --an intracellular structural motif that immediately follows the activation gate S6 helix --is responsible for Mg(2+)-dependent activation. Mutations that impair or abolish Mg(2+) sensitivity do not affect Ca(2+) sensitivity, and vice versa. These results indicate distinct structural pathways for Mg(2+)- and Ca(2+)-dependent activation and suggest a possible mechanism for the coupling between Mg(2+) binding and channel opening."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature00941"xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature00941"xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Shi J."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Shi J."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Qin J."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Qin J."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Yang Y."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Yang Y."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Hu L."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Hu L."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Cui J."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Cui J."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Chaturvedi N."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Chaturvedi N."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Krishnamoorthy G."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Krishnamoorthy G."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Harilal D."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/author | "Harilal D."xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/12192410 | http://purl.uniprot.org/core/name | "Nature"xsd:string |