| http://purl.uniprot.org/citations/12198487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12198487 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12198487 | http://www.w3.org/2000/01/rdf-schema#comment | "The execution of apoptosis or programmed cell death comprises both caspase-dependent and caspase-independent processes. Apoptosis inducing factor (AIF) was identified as a major player in caspase-independent cell death. It induces chromatin condensation and initial DNA cleavage via an unknown molecular mechanism. Here we report the crystal structure of human AIF at 1.8 A resolution. The structure reveals the presence of a strong positive electrostatic potential at the AIF surface, although the calculated isoelectric point for the entire protein is neutral. We show that recombinant AIF interacts with DNA in a sequence-independent manner. In addition, in cells treated with an apoptotic stimulus, endogenous AIF becomes co-localized with DNA at an early stage of nuclear morphological changes. Structure-based mutagenesis shows that DNA-binding defective mutants of AIF fail to induce cell death while retaining nuclear translocation. The potential DNA-binding site identified from mutagenesis also coincides with computational docking of a DNA duplex. These observations suggest that AIF-induced nuclear apoptosis requires a direct interaction with DNA."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.org/dc/terms/identifier | "doi:10.1038/nsb836"xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.org/dc/terms/identifier | "doi:10.1038/nsb836"xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Jiang S."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Jiang S."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Wu H."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Wu H."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Ye H."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Ye H."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Kroemer G."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Kroemer G."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Garrido C."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Garrido C."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Cande C."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Cande C."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Stephanou N.C."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Stephanou N.C."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Daugas E."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Daugas E."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Gurbuxani S."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Gurbuxani S."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Larochette N."xsd:string |
| http://purl.uniprot.org/citations/12198487 | http://purl.uniprot.org/core/author | "Larochette N."xsd:string |