| http://purl.uniprot.org/citations/12200420 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12200420 | http://www.w3.org/2000/01/rdf-schema#comment | "Pit-1 stimulates the expression of growth hormone, prolactin, and thyrotropin beta subunit genes. Consequently, abnormality of the Pit-1 gene results in combined pituitary hormone deficiency (CPHD). In this study, we analyzed the function of Pit-1 with a mutation (proline to leucine at codon 24) in the transactivation domain, P24L, which has a normal POU domain important for binding to DNA, because this mutation had been reported in a patient with CPHD. We found that codon 24 proline in the transactivation domain as well as the POU domain of Pit-1 was crucial to recruit coactivator CREB-binding protein (CBP) in the cultured cells. P24L completely lost the responsiveness to cAMP to stimulate the expression of the Pit-1-targeted genes. Furthermore, CBP and Pit-1, but not P24L, markedly enhanced the expression of the Pit-1-targeted gene to cAMP, and adenovirus E1a that binds to CBP and abrogates its function blocked the induction by cAMP of Pit-1-stimulated gene transcription in the pituitary-derived GH3 cells. These results suggest that CBP and proline at codon 24 in the transactivation domain of Pit-1 are important for the cAMP-induced activation of Pit-1-targeted genes. However, P24L maintained basal transcriptional activity, suggesting that CBP is unlikely to be an essential coactivator for Pit-1."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m202991200"xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/author | "Iida K."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/author | "Yagita K."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/author | "Kaji H."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/author | "Chihara K."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/author | "Okamura H."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/author | "Kishimoto M."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/author | "Okimura Y."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/author | "Fumoto M."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/author | "Iguchi G."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/pages | "45141-45148"xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/title | "Novel function of the transactivation domain of a pituitary-specific transcription factor, Pit-1."xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/12200420 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12200420 |
| http://purl.uniprot.org/citations/12200420 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12200420 |
| http://purl.uniprot.org/uniprot/#_Q16223-mappedCitation-12200420 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12200420 |
| http://purl.uniprot.org/uniprot/#_P28069-mappedCitation-12200420 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12200420 |
| http://purl.uniprot.org/uniprot/Q16223 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12200420 |
| http://purl.uniprot.org/uniprot/P28069 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12200420 |