| http://purl.uniprot.org/citations/12206660 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12206660 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12206660 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/12206660 | http://www.w3.org/2000/01/rdf-schema#comment | "Heme A, an obligatory cofactor in eukaryotic cytochrome c oxidase, is produced from heme B (protoheme) via two enzymatic reactions catalyzed by heme O synthase and heme A synthase. Heme O synthase is responsible for the addition of a farnesyl moiety, while heme A synthase catalyzes the oxidation of a methyl substituent to an aldehyde. We have cloned the heme O synthase and heme A synthase genes from Bacillus subtilis (ctaB and ctaA) and overexpressed them in Escherichia coli to probe the oxidative mechanism of heme A synthase. Because E. coli does not naturally produce or utilize heme A, this strategy effectively decoupled heme A biosynthesis from the native electron transfer pathway and heme A transport, allowing us to observe two previously unidentified hemes. We utilized HPLC, UV/visible spectroscopy, and tandem mass spectrometry to identify these novel hemes as derivatives of heme O containing an alcohol or a carboxylate moiety at position C8 on pyrrole ring D. We interpret these derivatives to be the putative alcohol intermediate and an overoxidized byproduct of heme A synthase. Because we have shown that all hemes produced by heme A synthase require O(2) for their synthesis, we propose that heme A synthase catalyzes the oxidation of the C8 methyl to an aldehyde group via two discrete monooxygenase reactions."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi0203536"xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi0203536"xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/author | "Hegg E.L."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/author | "Hegg E.L."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/author | "Brown K.R."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/author | "Brown K.R."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/author | "Allan B.M."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/author | "Allan B.M."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/author | "Do P."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/author | "Do P."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/pages | "10906-10913"xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/pages | "10906-10913"xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/title | "Identification of novel hemes generated by heme A synthase: evidence for two successive monooxygenase reactions."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/title | "Identification of novel hemes generated by heme A synthase: evidence for two successive monooxygenase reactions."xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/volume | "41"xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://purl.uniprot.org/core/volume | "41"xsd:string |
| http://purl.uniprot.org/citations/12206660 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12206660 |