| http://purl.uniprot.org/citations/12207026 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12207026 | http://www.w3.org/2000/01/rdf-schema#comment | "Tumor necrosis factor alpha-convertase (TACE) is a metalloprotease-disintegrin involved in the ectodomain shedding of several proteins and is critical for proper murine development. TACE-mediated ectodomain shedding is regulated, and the cytoplasmic domain of TACE contains several potential signaling motifs, suggesting that this domain may play a role in regulating the metalloprotease activity. Here we report that the protein-tyrosine phosphatase PTPH1, which contains both a band 4.1 domain and a single PDZ domain, can interact with the cytoplasmic domain of TACE. The interaction was initially observed in a yeast two-hybrid screen and was confirmed using an in vitro binding assay and co-immunoprecipitations from eukaryotic cell extracts. The interaction is mediated via binding of the PDZ domain of PTPH1 to the COOH terminus of TACE. The latter represents a novel group I PDZ binding sequence characterized by a terminal cysteine residue. In co-expression experiments, significantly lower levels of TACE were observed in the presence of catalytically active forms of PTPH1 compared with catalytically inactive forms of PTPH1. Furthermore, phorbol ester-stimulated shedding of the TACE substrate tumor necrosis factor-alpha was decreased in cells expressing catalytically active PTPH1 compared with inactive PTPH1. Taken together, these results suggest that PTPH1 may be a negative regulator of TACE levels and function, and thus provide the first evidence for the regulation of TACE through a cytoplasmic protein."xsd:string |
| http://purl.uniprot.org/citations/12207026 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m207459200"xsd:string |
| http://purl.uniprot.org/citations/12207026 | http://purl.uniprot.org/core/author | "Zheng Y."xsd:string |
| http://purl.uniprot.org/citations/12207026 | http://purl.uniprot.org/core/author | "Blobel C.P."xsd:string |
| http://purl.uniprot.org/citations/12207026 | http://purl.uniprot.org/core/author | "Schlondorff J."xsd:string |
| http://purl.uniprot.org/citations/12207026 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12207026 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12207026 | http://purl.uniprot.org/core/pages | "42463-42470"xsd:string |
| http://purl.uniprot.org/citations/12207026 | http://purl.uniprot.org/core/title | "Evidence for regulation of the tumor necrosis factor alpha-convertase (TACE) by protein-tyrosine phosphatase PTPH1."xsd:string |
| http://purl.uniprot.org/citations/12207026 | http://purl.uniprot.org/core/volume | "277"xsd:string |
| http://purl.uniprot.org/citations/12207026 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12207026 |
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| http://purl.uniprot.org/uniprot/#_Q8N4S3-mappedCitation-12207026 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12207026 |