| http://purl.uniprot.org/citations/12210765 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12210765 | http://www.w3.org/2000/01/rdf-schema#comment | "Eukaryotic initiation factor 5A (eIF-5A) is the only protein in nature that contains hypusine, an unusual amino acid residue formed posttranslationally by deoxyhypusine synthase and deoxyhypusine hydroxylase. Although the eIF-5A gene is essential for cell survival and proliferation, the precise function and localization of eIF-5A remain unclear. In this study, we have determined the subcellular distribution of eIF-5A by indirect immunofluorescent staining and by direct visualization of green fluorescent protein tagged eIF-5A (GFP-eIF5A). Immunofluorescent staining of the formaldehyde-fixed cells showed that eIF-5A was present in both the nucleus and cytoplasm. Only the nuclear eIF-5A was resistant to Triton extraction. Direct visualization of GFP tagged eIF-5A in living cells revealed the same whole-cell distribution pattern. However, a fusion of an additional pyruvate kinase (PK) moiety into GFP-eIF-5A precluded the nuclear localization of GFP-PK-eIF-5A fusion protein. Fusion of the GFP-PK tag with three different domains of eIF-5A also failed to reveal any nuclear localization of the fusion proteins, suggesting the absence of receptor-mediated nuclear import. Using interspecies heterokaryon fusion assay, we could detect the nuclear export of GFP-Rev, but not of GFP-eIF-5A. The whole-cell distribution pattern of eIF-5A was recalcitrant to the treatments that included energy depletion, heat shock, and inhibition of transcription, translation, polyamine synthesis, or CRM1-dependent nuclear export. Collectively, our data indicate that eIF-5A gains nuclear entry via passive diffusion, but it does not undergo active nucleocytoplasmic shuttling."xsd:string |
| http://purl.uniprot.org/citations/12210765 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.10235"xsd:string |
| http://purl.uniprot.org/citations/12210765 | http://purl.uniprot.org/core/author | "Jao D.L."xsd:string |
| http://purl.uniprot.org/citations/12210765 | http://purl.uniprot.org/core/author | "Yu Chen K."xsd:string |
| http://purl.uniprot.org/citations/12210765 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12210765 | http://purl.uniprot.org/core/name | "J Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/12210765 | http://purl.uniprot.org/core/pages | "590-600"xsd:string |
| http://purl.uniprot.org/citations/12210765 | http://purl.uniprot.org/core/title | "Subcellular localization of the hypusine-containing eukaryotic initiation factor 5A by immunofluorescent staining and green fluorescent protein tagging."xsd:string |
| http://purl.uniprot.org/citations/12210765 | http://purl.uniprot.org/core/volume | "86"xsd:string |
| http://purl.uniprot.org/citations/12210765 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12210765 |
| http://purl.uniprot.org/citations/12210765 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12210765 |
| http://purl.uniprot.org/uniprot/P63241#attribution-6D901C95E3F6A96C923CCDFBD8B70C0B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12210765 |
| http://purl.uniprot.org/uniprot/O14980#attribution-6D901C95E3F6A96C923CCDFBD8B70C0B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12210765 |
| http://purl.uniprot.org/uniprot/#_B7Z8F1-mappedCitation-12210765 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12210765 |
| http://purl.uniprot.org/uniprot/#_P63241-mappedCitation-12210765 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12210765 |
| http://purl.uniprot.org/uniprot/#_Q8WXQ9-mappedCitation-12210765 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12210765 |
| http://purl.uniprot.org/uniprot/P63241 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12210765 |
| http://purl.uniprot.org/uniprot/B7Z8F1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12210765 |
| http://purl.uniprot.org/uniprot/Q8WXQ9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12210765 |