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http://purl.uniprot.org/citations/12217076http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12217076http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12217076http://www.w3.org/2000/01/rdf-schema#comment"The integration of light-harvesting chlorophyll proteins (LHCPs) into the thylakoid membrane requires the integral thylakoid membrane protein ALB3, a homologue of the bacterial cytoplasmic membrane protein YidC. In bacteria, YidC is associated with the SecY-translocase and facilitates the integration of Sec-dependent proteins into the plasma membrane. In addition, it is also involved in the insertion of Sec-independent proteins. In the present study we demonstrate, in Arabidopsis thaliana, that most ALB3 is a constituent of an oligomeric complex of approx. 180 kDa. In addition, we detected ALB3 in several higher-molecular-mass complexes (up to 700 kDa). Furthermore, we show that most ALB3 co-fractionates with cpSecY during gel-filtration analysis and blue native gel electrophoresis, suggesting an association of ALB3 with the cpSecY complex. A direct interaction of ALB3 with the cpSecY complex was demonstrated by co-immunoprecipitation experiments using digitonin-solubilized thylakoid membrane proteins and anti-cpSecY or anti-ALB3 antibodies. This result was further confirmed by electron microscopic co-immunolocalization of ALB3 and cpSecY. In addition, an association of ALB3 with the cpSecY complex was demonstrated directly by cross-linking experiments using the chemical cross-linker disuccinimidyl suberate."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.org/dc/terms/identifier"doi:10.1042/bj20021291"xsd:string
http://purl.uniprot.org/citations/12217076http://purl.org/dc/terms/identifier"doi:10.1042/bj20021291"xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/author"Carde J.P."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/author"Carde J.P."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/author"Droste Gen Helling I."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/author"Droste Gen Helling I."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/author"Klostermann E."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/author"Klostermann E."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/author"Schunemann D."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/author"Schunemann D."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/date"2002"xsd:gYear
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/name"Biochem. J."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/name"Biochem. J."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/pages"777-781"xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/pages"777-781"xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/title"The thylakoid membrane protein ALB3 associates with the cpSecY-translocase in Arabidopsis thaliana."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/title"The thylakoid membrane protein ALB3 associates with the cpSecY-translocase in Arabidopsis thaliana."xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/volume"368"xsd:string
http://purl.uniprot.org/citations/12217076http://purl.uniprot.org/core/volume"368"xsd:string
http://purl.uniprot.org/citations/12217076http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/12217076
http://purl.uniprot.org/citations/12217076http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/12217076