| http://purl.uniprot.org/citations/12221128 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12221128 | http://www.w3.org/2000/01/rdf-schema#comment | "Microtubule assembly is initiated by the gamma-tubulin ring complex (gamma-TuRC). In yeast, the microtubule is nucleated from gamma-TuRC anchored to the amino-terminus of the spindle pole body component Spc110p, which interacts with calmodulin (Cmd1p) at the carboxy-terminus. However, mammalian protein that anchors gamma-TuRC remains to be elucidated. A giant coiled-coil protein, CG-NAP (centrosome and Golgi localized PKN-associated protein), was localized to the centrosome via the carboxyl-terminal region. This region was found to interact with calmodulin by yeast two-hybrid screening, and it shares high homology with the carboxyl-terminal region of another centrosomal coiled-coil protein, kendrin. The amino-terminal region of either CG-NAP or kendrin indirectly associated with gamma-tubulin through binding with gamma-tubulin complex protein 2 (GCP2) and/or GCP3. Furthermore, endogenous CG-NAP and kendrin were coimmunoprecipitated with each other and with endogenous GCP2 and gamma-tubulin, suggesting that CG-NAP and kendrin form complexes and interact with gamma-TuRC in vivo. These proteins were localized to the center of microtubule asters nucleated from isolated centrosomes. Pretreatment of the centrosomes by antibody to CG-NAP or kendrin moderately inhibited the microtubule nucleation; moreover, the combination of these antibodies resulted in stronger inhibition. These results imply that CG-NAP and kendrin provide sites for microtubule nucleation in the mammalian centrosome by anchoring gamma-TuRC."xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e02-02-0112"xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://purl.uniprot.org/core/author | "Ono Y."xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://purl.uniprot.org/core/author | "Nishimura T."xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://purl.uniprot.org/core/author | "Takahashi M."xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://purl.uniprot.org/core/author | "Mukai H."xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://purl.uniprot.org/core/author | "Yamagiwa A."xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12221128 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://purl.uniprot.org/core/pages | "3235-3245"xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://purl.uniprot.org/core/title | "Centrosomal proteins CG-NAP and kendrin provide microtubule nucleation sites by anchoring gamma-tubulin ring complex."xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://purl.uniprot.org/core/volume | "13"xsd:string |
| http://purl.uniprot.org/citations/12221128 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12221128 |
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