| http://purl.uniprot.org/citations/12354761 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12354761 | http://www.w3.org/2000/01/rdf-schema#comment | "Regulator of G protein signaling (RGS) proteins contain an RGS domain that inhibits G(alpha) signaling by activating G(alpha) GTPase activity. Certain RGS proteins also contain a Ggamma-like (GGL) domain and a poorly characterized but conserved N-terminal region. We assessed the functions of these subregions in the Caenorhabditis elegans RGS proteins EGL-10 and EAT-16, which selectively inhibit GOA-1 (G(alpha)(o)) and EGL-30 (G(alpha)(q)), respectively. Using transgenes in C. elegans, we expressed EGL-10, EAT-16, their subregions, or EGL-10/EAT-16 chimeras. The chimeras showed that the GGL/RGS region of either protein can act on either GOA-1 or EGL-30 and that a key factor determining G(alpha) target selectivity is the manner in which the N-terminal and GGL/RGS regions are linked. We also found that coexpressing N-terminal and GGL/RGS fragments of EGL-10 gave full EGL-10 activity, whereas either fragment alone gave little activity. Biochemical analysis showed that coexpressing the two fragments caused both to increase in abundance and also caused the GGL/RGS fragment to move to the membrane, where the N-terminal fragment is localized. By coimmunoprecipitation, we found that the N-terminal fragment complexes with the C-terminal fragment and its associated Gbeta subunit, GPB-2. We conclude that the N-terminal region directs inhibition of G(alpha) signaling by forming a complex with the GGL/RGS region and affecting its stability, membrane localization, and G(alpha) target specificity."xsd:string |
| http://purl.uniprot.org/citations/12354761 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m208186200"xsd:string |
| http://purl.uniprot.org/citations/12354761 | http://purl.uniprot.org/core/author | "Koelle M.R."xsd:string |
| http://purl.uniprot.org/citations/12354761 | http://purl.uniprot.org/core/author | "Patikoglou G.A."xsd:string |
| http://purl.uniprot.org/citations/12354761 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12354761 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12354761 | http://purl.uniprot.org/core/pages | "47004-47013"xsd:string |
| http://purl.uniprot.org/citations/12354761 | http://purl.uniprot.org/core/title | "An N-terminal region of Caenorhabditis elegans RGS proteins EGL-10 and EAT-16 directs inhibition of G(alpha)o versus G(alpha)q signaling."xsd:string |
| http://purl.uniprot.org/citations/12354761 | http://purl.uniprot.org/core/volume | "277"xsd:string |
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