| http://purl.uniprot.org/citations/12356940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12356940 | http://www.w3.org/2000/01/rdf-schema#comment | "Type I inositol 1,4,5-trisphosphate-sensitive receptors (InsP(3)R) are expressed in human oocytes and may be involved in operating the Ca(2+) release triggered by the fertilizing sperm. This study examines the contribution of type I InsP(3)R in operating Ca(2+) release in human oocytes secondary to InsP(3) itself, using a specific function-blocking antibody in conjunction with photolytic release of microinjected InsP(3). Intracellular Ca(2+) responses were assessed in oocytes microinjected with only caged InsP(3) in experiment set A, while in experiment sets B and C, sibling oocytes were injected with caged InsP(3) and the blocking antibody or a corresponding volume of medium, prior to flash photolysis. In experiment set C, certain fertilization-related phenomena (cortical granule exocytosis and chromatin configurations) were assessed using optical sections and three-dimensional image reconstructions obtained from a confocal laser scanning microscope. In experiment set A, photolytic release of InsP(3) triggered a Ca(2+) response (increase from approximately 100 to 220 nmol/l followed by an exponential recovery, n = 8) and a wave in the oocytes that spread from the stimulation point to the opposite pole. In set B, photolytic InsP(3) release generated Ca(2+) responses in control oocytes (n = 9), but not in the antibody-injected oocytes (n = 7). In set C, cortical granule exocytosis and anaphase chromosome configurations were noted in the control oocytes after flash photolysis (n = 6). These changes were completely absent in antibody injected oocytes as their cortical granules were intact and the chromosomes were in metaphase. These oocytes had also lacked Ca(2+) responses as in set B (n = 5). This study demonstrates the functional presence of type I InsP(3)R-operated Ca(2+) channels in human oocytes and further suggests an active role of InsP(3) in triggering the Ca(2+) rise and secondary activation phenomena at fertilization."xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.org/dc/terms/identifier | "doi:10.1093/molehr/8.10.912"xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/author | "Mikoshiba K."xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/author | "Van Oostveldt P."xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/author | "Leybaert L."xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/author | "Diamond M.P."xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/author | "Dhont M."xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/author | "Goud A.P."xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/author | "Goud P.T."xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/name | "Mol Hum Reprod"xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/pages | "912-918"xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/title | "Inositol 1,4,5-trisphosphate receptor function in human oocytes: calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP(3) are blocked by a specific antibody to the type I receptor."xsd:string |
| http://purl.uniprot.org/citations/12356940 | http://purl.uniprot.org/core/volume | "8"xsd:string |
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