| http://purl.uniprot.org/citations/12372257 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12372257 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12372257 | http://www.w3.org/2000/01/rdf-schema#comment | "The small GTP binding protein ARF has been implicated in budding clathrin-coated vesicles (CCVs) from Golgi and endosomal membranes. An arf1 synthetic lethal screen identified DRS2/SWA3 along with a clathrin heavy-chain conditional allele (chc1-5/swa5-1) and SWA2, encoding the yeast auxilin-like protein involved in uncoating CCVs. Drs2p/Swa3p is a P-type ATPase and a potential aminophospholipid translocase that localizes to the trans-Golgi network (TGN) in yeast. Genetic and phenotypic analyses of drs2Delta mutants suggested that Drs2p was required for clathrin function. To address a potential role for Drs2p in CCV formation from the TGN in vivo, we have performed epistasis analyses between drs2 and mutations that cause accumulation of distinct populations of post-Golgi vesicles. We find that Drs2p is required to form a specific class of secretory vesicles that accumulate when the actin cytoskeleton is disrupted. Accumulation of these vesicles also requires clathrin and is perturbed by mutation of AP-1, but not AP-2, AP-3, or GGA adaptins. Most of the accumulated vesicles are uncoated; however, clathrin coats can be partially stabilized on these vesicles by deletion of SWA2. These data provide the first in vivo evidence for an integral membrane protein requirement in forming CCVs."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0960-9822(02)01148-x"xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0960-9822(02)01148-x"xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Liu K."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Liu K."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Graham T.R."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Graham T.R."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Hua Z."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Hua Z."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Ingram M.F."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Ingram M.F."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Chen S.I."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Chen S.I."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Gall W.E."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Gall W.E."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Geething N.C."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/author | "Geething N.C."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/name | "Curr. Biol."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/name | "Curr. Biol."xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/pages | "1623-1627"xsd:string |
| http://purl.uniprot.org/citations/12372257 | http://purl.uniprot.org/core/pages | "1623-1627"xsd:string |