| http://purl.uniprot.org/citations/12374739 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12374739 | http://www.w3.org/2000/01/rdf-schema#comment | "Microinjection in mouse eggs of tr-kit, a truncated form of the c-kit tyrosine kinase present in mouse spermatozoa, causes resumption of meiosis through activation of phospholipase Cgamma1 (PLCgamma1) and Ca(2+) mobilization from intracellular stores. We show that the Src-like kinase Fyn phosphorylates Tyr161 in tr-kit and that this residue is essential for tr-kit function. Fyn is localized in the cortex region underneath the plasma membrane in mouse oocytes. Using several approaches, we demonstrate that Fyn associates with tr-kit and that the interaction requires Tyr161. The interaction between tr-kit and Fyn triggers activation of the kinase as monitored by both autophosphorylation and phosphorylation of PLCgamma1. Co-injection of tr-kit with the SH2 domain of Fyn, or pre-treatment with a Fyn inhibitor, impairs oocyte activation, suggesting that activation of Fyn by tr-kit also occurs in vivo. Finally, microinjection of constitutively active Fyn triggers oocyte activation downstream of tr-kit but still requires PLC activity. We suggest that the mechanism by which tr-kit triggers resumption of meiosis of mouse eggs requires a functional interaction with Fyn and phosphorylation of PLCgamma1."xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/cdf553"xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/author | "Geremia R."xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/author | "Rossi P."xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/author | "Barchi M."xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/author | "Bevilacqua A."xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/author | "Paronetto M.P."xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/author | "Sette C."xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/pages | "5386-5395"xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/title | "Tr-kit-induced resumption of the cell cycle in mouse eggs requires activation of a Src-like kinase."xsd:string |
| http://purl.uniprot.org/citations/12374739 | http://purl.uniprot.org/core/volume | "21"xsd:string |
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