| http://purl.uniprot.org/citations/12376668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12376668 | http://www.w3.org/2000/01/rdf-schema#comment | "The regulation of intracellular Ca(2+) levels is achieved in part by high-capacity vacuolar Ca(2+)/H(+) antiporters. An N-terminal regulatory region (NRR) on the Arabidopsis Ca(2+)/H(+) antiporter CAX1 (cation exchanger 1) has been shown previously to regulate Ca(2+) transport by a mechanism of N-terminal auto-inhibition. Here, we examine the regulation of other CAX transporters, both within Arabidopsis and from another plant, mung bean (Vigna radiata), to ascertain if this mechanism is commonly used among Ca(2+)/H(+) antiporters. Biochemical analysis of mung bean VCAX1 expressed in yeast (Saccharomyces cerevisiae) showed that N-terminal truncated VCAX1 had approximately 70% greater antiport activity compared with full-length VCAX1. A synthetic peptide corresponding to the NRR of CAX1, which can strongly inhibit Ca(2+) transport by CAX1, could not dramatically inhibit Ca(2+) transport by truncated VCAX1. The N terminus of Arabidopsis CAX3 was also shown to contain an NRR. Additions of either the CAX3 or VCAX1 regulatory regions to the N terminus of an N-terminal truncated CAX1 failed to inhibit CAX1 activity. When fused to N-terminal truncated CAX1, both the CAX3 and VCAX1 regulatory regions could only auto-inhibit CAX1 after mutagenesis of specific amino acids within this NRR region. These findings demonstrate that N-terminal regulation is present in other plant CAX transporters, and suggest distinct regulatory features among these transporters."xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.008193"xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://purl.uniprot.org/core/author | "Pittman J.K."xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://purl.uniprot.org/core/author | "Hirschi K.D."xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://purl.uniprot.org/core/author | "Shigaki T."xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://purl.uniprot.org/core/author | "Sreevidya C.S."xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://purl.uniprot.org/core/author | "Ueoka-Nakanishi H."xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12376668 | http://purl.uniprot.org/core/name | "Plant Physiol"xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://purl.uniprot.org/core/pages | "1054-1062"xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://purl.uniprot.org/core/title | "Distinct N-terminal regulatory domains of Ca(2+)/H(+) antiporters."xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://purl.uniprot.org/core/volume | "130"xsd:string |
| http://purl.uniprot.org/citations/12376668 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12376668 |
| http://purl.uniprot.org/citations/12376668 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12376668 |
| http://purl.uniprot.org/uniprot/#_A0A1I9LSS9-mappedCitation-12376668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12376668 |
| http://purl.uniprot.org/uniprot/#_B9DFT4-mappedCitation-12376668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12376668 |
| http://purl.uniprot.org/uniprot/#_F4IS06-mappedCitation-12376668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12376668 |
| http://purl.uniprot.org/uniprot/#_Q39253-mappedCitation-12376668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12376668 |
| http://purl.uniprot.org/uniprot/#_P25296-mappedCitation-12376668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12376668 |
| http://purl.uniprot.org/uniprot/#_M5BFB1-mappedCitation-12376668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12376668 |
| http://purl.uniprot.org/uniprot/#_P38929-mappedCitation-12376668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12376668 |
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| http://purl.uniprot.org/uniprot/Q99385 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12376668 |