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http://purl.uniprot.org/citations/12379656 | http://www.w3.org/2000/01/rdf-schema#comment | "We have recently reported the crystal structure of the accessory subunit of mitochondrial DNA polymerase, pol gammaB, and identified a region of the protein involved in DNA binding. The DNA employed in previous studies was presumed to be single-stranded, because it was generated by single-sided PCR. Further characterization of this DNA indicated that, due to a strand transfer event during synthesis by single-sided PCR, the DNA adopts a double-stranded hairpin conformation under native conditions. We used a series of double- and single-stranded oligonucleotides of different lengths to confirm that human pol gammaB prefers to bind double-stranded DNA longer than 40 bp with little apparent sequence specificity. Site-specific deletion mutagenesis identified clusters of basic residues in two surface loops required for DNA binding located on opposite sides of the symmetrical pol gammaB dimer. A heterodimer of pol gammaB that contains one mutant and one wild-type DNA binding region was shown to be unable to bind double-stranded DNA, suggesting that a single DNA molecule must contact both DNA binding sites in the pol gammaB dimer. The ability to bind double-stranded DNA is not essential for pol gammaB stimulation of pol gammaA activity in vitro, but may play a role in DNA replication or repair."xsd:string |
http://purl.uniprot.org/citations/12379656 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m207030200"xsd:string |
http://purl.uniprot.org/citations/12379656 | http://purl.uniprot.org/core/author | "Bogenhagen D.F."xsd:string |
http://purl.uniprot.org/citations/12379656 | http://purl.uniprot.org/core/author | "Carrodeguas J.A."xsd:string |
http://purl.uniprot.org/citations/12379656 | http://purl.uniprot.org/core/author | "Pinz K.G."xsd:string |
http://purl.uniprot.org/citations/12379656 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
http://purl.uniprot.org/citations/12379656 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
http://purl.uniprot.org/citations/12379656 | http://purl.uniprot.org/core/pages | "50008-50014"xsd:string |
http://purl.uniprot.org/citations/12379656 | http://purl.uniprot.org/core/title | "DNA binding properties of human pol gammaB."xsd:string |
http://purl.uniprot.org/citations/12379656 | http://purl.uniprot.org/core/volume | "277"xsd:string |
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