| http://purl.uniprot.org/citations/12383517 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12383517 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12383517 | http://www.w3.org/2000/01/rdf-schema#comment | "Glucokinase gene (HPGLK1) was cloned from a methylotrophic yeast Hansenula polymorpha by complementation of glucose-phosphorylation deficiency in a H. polymorpha double kinase-negative mutant A31-10 by a genomic library. An open reading frame of 1416 nt encoding a 471-amino-acid protein with calculated molecular weight 51.6 kDa was characterized in the genomic insert of the plasmid pH3. The protein sequence deduced from HPGLK1 exhibited 55 and 46% identity with glucokinases from Saccharomyces cerevisiae and Aspergillus niger, respectively. The enzyme phosphorylated glucose, mannose and 2-deoxyglucose, but not fructose. Transformation of HPGLK1 into A31-10 restored glucose repression of alcohol oxidase and catalase in the mutant. Transformation of HPGLK1 into S. cerevisiae triple kinase-negative mutant DFY632 showed that H. polymorpha glucokinase cannot transmit the glucose repression signal in S. CEREVSIAE: synthesis of invertase and maltase in respective transformants was insensitive to glucose repression similarly to S. cerevisiae DFY568 possessing only glucokinase."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.org/dc/terms/identifier | "doi:10.1016/S0378-1119(02)00859-4"xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0378-1119(02)00859-4"xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/author | "Alamae T."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/author | "Alamae T."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/author | "Jarviste A."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/author | "Jarviste A."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/author | "Karp H."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/author | "Karp H."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/author | "Kotka P."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/author | "Kotka P."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/author | "Laht S."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/author | "Laht S."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/pages | "195-203"xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/pages | "195-203"xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/title | "Cloning and characterization of glucokinase from a methylotrophic yeast Hansenula polymorpha: different effects on glucose repression in H. polymorpha and Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/title | "Cloning and characterization of glucokinase from a methylotrophic yeast Hansenula polymorpha: different effects on glucose repression in H. polymorpha and Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/volume | "296"xsd:string |
| http://purl.uniprot.org/citations/12383517 | http://purl.uniprot.org/core/volume | "296"xsd:string |