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| http://purl.uniprot.org/citations/12393745 | http://www.w3.org/2000/01/rdf-schema#comment | "The erythroid-specific isoform of 5-aminolevulinate synthase (ALAS2) catalyzes the rate-limiting step in heme biosynthesis. The hypoxia-inducible factor-1 (HIF-1) transcriptionally up-regulates erythropoietin, transferrin, and transferrin receptor, leading to increased erythropoiesis and hematopoietic iron supply. To test the hypothesis that ALAS2 expression might be regulated by a similar mechanism, we exposed murine erythroleukemia cells to hypoxia (1% O(2)) and found an up to 3-fold up-regulation of ALAS2 mRNA levels and an increase in cellular heme content. A fragment of the ALAS2 promoter ranging from -716 to +1 conveyed hypoxia responsiveness to a heterologous luciferase reporter gene construct in transiently transfected HeLa cells. In contrast, iron depletion, known to induce HIF-1 activity but inhibit ALAS2 translation, did not increase ALAS2 promoter activity. Mutation of a previously predicted HIF-1-binding site (-323/-318) within this promoter fragment and DNA-binding assays revealed that hypoxic up-regulation is independent of this putative HIF-1 DNA-binding site."xsd:string |
| http://purl.uniprot.org/citations/12393745 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2002-03-0773"xsd:string |
| http://purl.uniprot.org/citations/12393745 | http://purl.uniprot.org/core/author | "Wenger R.H."xsd:string |
| http://purl.uniprot.org/citations/12393745 | http://purl.uniprot.org/core/author | "Ferreira G.C."xsd:string |
| http://purl.uniprot.org/citations/12393745 | http://purl.uniprot.org/core/author | "Gassmann M."xsd:string |
| http://purl.uniprot.org/citations/12393745 | http://purl.uniprot.org/core/author | "Hofer T."xsd:string |
| http://purl.uniprot.org/citations/12393745 | http://purl.uniprot.org/core/author | "Kramer M.F."xsd:string |
| http://purl.uniprot.org/citations/12393745 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12393745 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/12393745 | http://purl.uniprot.org/core/pages | "348-350"xsd:string |
| http://purl.uniprot.org/citations/12393745 | http://purl.uniprot.org/core/title | "Hypoxic up-regulation of erythroid 5-aminolevulinate synthase."xsd:string |
| http://purl.uniprot.org/citations/12393745 | http://purl.uniprot.org/core/volume | "101"xsd:string |
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