| http://purl.uniprot.org/citations/12409283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12409283 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12409283 | http://www.w3.org/2000/01/rdf-schema#comment | "Human trophoblasts depend on the supply of external precursors, such as dehydroepiandrosterone-3-sulfate (DHEA-S) and 16 alpha-OH-DHEA-S, for synthesis of estrogens. The aim of the present study was to characterize the uptake of DHEA-S by isolated mononucleated trophoblasts (MT) and to identify the involved transporter polypeptides. The kinetic analysis of DHEA-(35)S uptake by MT revealed a saturable uptake mechanism (K(m) = 26 microM, V(max) = 428 pmol x mg protein(-1) x min(-1)), which was superimposed by a nonsaturable uptake mechanism (diffusion constant = 1.2 microl x mg protein(-1) x min(-1)). Uptake of [(3)H]DHEA-S by MT was Na(+) dependent and inhibited by sulfobromophthalein (BSP), steroid sulfates, and probenecid, but not by steroid glucuronides, unconjugated steroids, conjugated bile acids, ouabain, p-aminohippurate (PAH), and bumetanide. MT took up [(35)S]BSP, [(3)H]estrone-sulfate, but not (3)H-labeled ouabain, estradiol-17beta-glucuronide, taurocholate, and PAH. RT-PCR revealed that the organic anion-transporting polypeptides OATP-B, -D, -E, and the organic anion transporter OAT-4 are highly expressed, and that OATP-A, -C, -8, OAT-3, and Na(+)-taurocholate cotransporting polypeptide (NTCP) are not or are only lowly expressed in term placental tissue and freshly isolated and cultured trophoblasts. Immunohistochemistry of first- and third-trimester placenta detected OAT-4 on cytotrophoblast membranes and at the basal surface of the syncytiotrophoblast. Our results indicate that uptake of steroid sulfates by isolated MT is mediated by OATP-B and OAT-4 and suggest a physiological role of both carrier proteins in placental uptake of fetal-derived steroid sulfates."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpendo.00257.2002"xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpendo.00257.2002"xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/author | "St-Pierre M.V."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/author | "St-Pierre M.V."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/author | "Bahn A."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/author | "Bahn A."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/author | "Hantschmann P."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/author | "Hantschmann P."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/author | "Pihusch M."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/author | "Pihusch M."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/author | "Ugele B."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/author | "Ugele B."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/name | "Am. J. Physiol."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/name | "Am. J. Physiol."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/pages | "E390-E398"xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/pages | "E390-E398"xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/title | "Characterization and identification of steroid sulfate transporters of human placenta."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/title | "Characterization and identification of steroid sulfate transporters of human placenta."xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/volume | "284"xsd:string |
| http://purl.uniprot.org/citations/12409283 | http://purl.uniprot.org/core/volume | "284"xsd:string |