| http://purl.uniprot.org/citations/12414733 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12414733 | http://www.w3.org/2000/01/rdf-schema#comment | "It is well established that the template for telomeric DNA synthesis is provided by the RNA subunit of telomerase; however, the additional functions provided by most of the rest of the RNA (>1000 nucleotides in budding yeast) are largely unknown. By alignment of telomerase RNAs of Saccharomyces cerevisiae and six Kluyveromyces species followed by mutagenesis of the S. cerevisiae RNA, we found a conserved region that is essential for telomere maintenance. Phylogenetic analysis and computer folding revealed that this region is conserved not only in primary nucleotide sequence but also in secondary structure. A common bulged-stem structure was predicted in all seven yeast species. Mutational analysis showed the structure to be essential for telomerase function. Suppression of bulged-stem mutant phenotypes by overexpression of Est1p and loss of co-immunoprecipitation of the mutant RNAs with Est1p indicated that this bulged stem is necessary for association of Est1p, a telomerase regulatory subunit. Est1p in yeast extract bound specifically to a small RNA containing the bulged stem, suggesting a direct interaction. We propose that this RNA structure links the enzymatic core of telomerase with Est1p, thereby allowing Est1p to recruit or activate telomerase at the telomere."xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1029302"xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://purl.uniprot.org/core/author | "Cech T.R."xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://purl.uniprot.org/core/author | "Blackburn E.H."xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://purl.uniprot.org/core/author | "Seto A.G."xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://purl.uniprot.org/core/author | "Tzfati Y."xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://purl.uniprot.org/core/author | "Livengood A.J."xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12414733 | http://purl.uniprot.org/core/name | "Genes Dev"xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://purl.uniprot.org/core/pages | "2800-2812"xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://purl.uniprot.org/core/title | "A bulged stem tethers Est1p to telomerase RNA in budding yeast."xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/12414733 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12414733 |
| http://purl.uniprot.org/citations/12414733 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12414733 |
| http://purl.uniprot.org/uniprot/P17214#attribution-DE71DF938B77E3A4289DE6F2FC5D9AA9 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12414733 |
| http://purl.uniprot.org/uniprot/#_P17214-mappedCitation-12414733 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12414733 |
| http://purl.uniprot.org/uniprot/P17214 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12414733 |