| http://purl.uniprot.org/citations/12416028 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12416028 | http://www.w3.org/2000/01/rdf-schema#comment | "Tumor cell adhesion to extracellular matrix (ECM) and its stabilization are important determinants in metastasis formation, and they are mediated, in part, by integrins and regulated by a variety of protein kinases. Protein tyrosine kinase pp60c-src is found in adhesion-dependent focal adhesion plaques where it may regulate different integrin-mediated signaling cascades. Using human HT-29 colon carcinoma cells stably transfected with pp60c-src--specific antisense oligonucleotides (HT-29AS15), we investigated the role of pp60c-src in integrin-mediated adhesion and its stabilization to ECM components collagen I or IV under static and laminar fluid flow conditions. Under static adhesion conditions transfection of pp60c-src antisense oligonucleotides did not modify adhesive properties. Phosphorylation of focal adhesion kinase (FAK) and paxillin induced by static adhesion to collagen I or IV were similar in HT-29P and HT-29AS15 cells. However, using hydrodynamic conditions in a laminar flow chamber we found a slight reduction in early adhesion events and an even greater difference in adhesion stabilization rates (ASRs). The transfected cells showed a significant reduction in their ability to withstand shear forces and stabilize adhesive bounds. These changes correlated with the cellular expression levels of pp60c-src. Our results suggest that pp60c-src may be involved in stabilization of dynamic HT-29 cell adhesion to ECM components, and this kinase appears to be part of a mechanosensory protein complex during integrin-mediated cell adhesion."xsd:string |
| http://purl.uniprot.org/citations/12416028 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1359-4117.2002.01051.x"xsd:string |
| http://purl.uniprot.org/citations/12416028 | http://purl.uniprot.org/core/author | "Gallick G.E."xsd:string |
| http://purl.uniprot.org/citations/12416028 | http://purl.uniprot.org/core/author | "Nicolson G.L."xsd:string |
| http://purl.uniprot.org/citations/12416028 | http://purl.uniprot.org/core/author | "Haier J."xsd:string |
| http://purl.uniprot.org/citations/12416028 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12416028 | http://purl.uniprot.org/core/name | "J Exp Ther Oncol"xsd:string |
| http://purl.uniprot.org/citations/12416028 | http://purl.uniprot.org/core/pages | "237-245"xsd:string |
| http://purl.uniprot.org/citations/12416028 | http://purl.uniprot.org/core/title | "Src protein kinase pp60c-src influences adhesion stabilization of HT-29 colon carcinoma cells to extracellular matrix components under dynamic conditions of laminar flow."xsd:string |
| http://purl.uniprot.org/citations/12416028 | http://purl.uniprot.org/core/volume | "2"xsd:string |
| http://purl.uniprot.org/citations/12416028 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12416028 |
| http://purl.uniprot.org/citations/12416028 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12416028 |
| http://purl.uniprot.org/uniprot/#_Q14925-mappedCitation-12416028 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12416028 |
| http://purl.uniprot.org/uniprot/#_P12931-mappedCitation-12416028 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12416028 |
| http://purl.uniprot.org/uniprot/#_Q71UK5-mappedCitation-12416028 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12416028 |
| http://purl.uniprot.org/uniprot/#_Q9H7V3-mappedCitation-12416028 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12416028 |
| http://purl.uniprot.org/uniprot/P12931 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12416028 |
| http://purl.uniprot.org/uniprot/Q9H7V3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12416028 |
| http://purl.uniprot.org/uniprot/Q71UK5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12416028 |
| http://purl.uniprot.org/uniprot/Q14925 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12416028 |