| http://purl.uniprot.org/citations/12417422 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12417422 | http://www.w3.org/2000/01/rdf-schema#comment | "Alpha-mannosidase IIx (MX) is an enzyme closely related to the Golgi N-glycan processing enzyme alpha-mannosidase II (MII). The enzymatic activity of MX in vitro is minimal. Therefore, the in vivo role of MX in N-glycan processing is as yet unclear. The targeted disruption of the gene encoding MX in the mouse resulted in an obvious phenotype, i.e., MX-deficient males were found to be infertile. Testes from homozygous mutant male mice are smaller than those from wild-type or heterozygous littermates. Histology of the MX null mouse testis showed significant reduction of spermatogenic cells in the seminiferous tubules. Electron microscopy showed that prominent intercellular spaces surround MX-deficient spermatogenic cells, suggesting a failure of germ cell adhesion to Sertoli cells. Quantitative structural analyses of N-glycans from wild-type and MX-deficient mouse testis showed that wild-type testes contain GlcNAc-terminated complex type N-glycans, while they are significantly reduced in MX-deficient mutant testis. An in vitro assay for adhesion of spermatogenic cells to Sertoli cells was carried out. By testing the effect of each purified N-glycan oligosaccharide, it was demonstrated that a GlcNAc-terminated tri-antennary, fucosylated N-glycan has an activity on the adhesion between germ cells and Sertoli cells. Thus, the targeted disruption of the gene encoding MX uncovered a novel carbohydrate recognition system in a biologically important process, spermatogenesis."xsd:string |
| http://purl.uniprot.org/citations/12417422 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0304-4165(02)00407-5"xsd:string |
| http://purl.uniprot.org/citations/12417422 | http://purl.uniprot.org/core/author | "Fukuda M.N."xsd:string |
| http://purl.uniprot.org/citations/12417422 | http://purl.uniprot.org/core/author | "Akama T.O."xsd:string |
| http://purl.uniprot.org/citations/12417422 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12417422 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/12417422 | http://purl.uniprot.org/core/pages | "382-387"xsd:string |
| http://purl.uniprot.org/citations/12417422 | http://purl.uniprot.org/core/title | "In vivo role of alpha-mannosidase IIx: ineffective spermatogenesis resulting from targeted disruption of the Man2a2 in the mouse."xsd:string |
| http://purl.uniprot.org/citations/12417422 | http://purl.uniprot.org/core/volume | "1573"xsd:string |
| http://purl.uniprot.org/citations/12417422 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12417422 |
| http://purl.uniprot.org/citations/12417422 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12417422 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RP42-mappedCitation-12417422 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RPB8-mappedCitation-12417422 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RNS8-mappedCitation-12417422 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RQ19-mappedCitation-12417422 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/#_Q8BRK9-mappedCitation-12417422 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/#_Q197W7-mappedCitation-12417422 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/#_Q3TSU5-mappedCitation-12417422 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/#_Q7TPQ0-mappedCitation-12417422 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/Q197W7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/Q8BRK9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/Q3TSU5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/A0A0U1RNS8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12417422 |
| http://purl.uniprot.org/uniprot/A0A0U1RPB8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12417422 |