| http://purl.uniprot.org/citations/12446729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12446729 | http://www.w3.org/2000/01/rdf-schema#comment | "Receptor tyrosine kinases activate mitogen-activated protein (MAP) kinases through Ras, Raf-1, and MEK. Receptor tyrosine kinases can be transactivated by G protein-coupled receptors coupling to G(i) and G(q). The human G protein-coupled serotonin receptors 5-HT(4(b)) and 5-HT(7(a)) couple to G(s) and elevate intracellular cAMP. Certain G(s)-coupled receptors have been shown to activate MAP kinases through a protein kinase A- and Rap1-dependent pathway. We report the activation of the extracellular signal-regulated kinases (ERKs) 1 and 2 (p44 and p42 MAP kinase) through the human serotonin receptors 5-HT(4(b)) and 5-HT(7(a)) in COS-7 and human embryonic kidney HEK293 cells. In transfected HEK293 cells, 5-HT-induced activation of ERK1/2 is sensitive to H89, which indicates a role for protein kinase A. The observed activation of ERK1/2 does not require transactivation of epidermal growth factor receptors. Furthermore, 5-HT induced activation of both Ras and Rap1. Whereas the presence of Rap1GAP1 did not influence the 5-HT-mediated activation of ERK1/2, the activation of ERK1/2 was abolished in the presence of dominant negative Ras (RasN17). ERK1/2 activation was reduced in the presence of "dominant negative" Raf1 (RafS621A) and slightly reduced by dominant negative B-Raf, indicating the involvement of one or more Raf isoforms. These findings suggest that activation of ERK1/2 through the human G(s)-coupled serotonin receptors 5-HT(4(b)) and 5-HT(7(a)) in HEK293 cells is dependent on Ras, but independent of Rap1."xsd:string |
| http://purl.uniprot.org/citations/12446729 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m206237200"xsd:string |
| http://purl.uniprot.org/citations/12446729 | http://purl.uniprot.org/core/author | "Levy F.O."xsd:string |
| http://purl.uniprot.org/citations/12446729 | http://purl.uniprot.org/core/author | "Hart K."xsd:string |
| http://purl.uniprot.org/citations/12446729 | http://purl.uniprot.org/core/author | "Norum J.H."xsd:string |
| http://purl.uniprot.org/citations/12446729 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12446729 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12446729 | http://purl.uniprot.org/core/pages | "3098-3104"xsd:string |
| http://purl.uniprot.org/citations/12446729 | http://purl.uniprot.org/core/title | "Ras-dependent ERK activation by the human G(s)-coupled serotonin receptors 5-HT4(b) and 5-HT7(a)."xsd:string |
| http://purl.uniprot.org/citations/12446729 | http://purl.uniprot.org/core/volume | "278"xsd:string |
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