| http://purl.uniprot.org/citations/12466525 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12466525 | http://www.w3.org/2000/01/rdf-schema#comment | "Previously, we have shown that the vimentin 3' untranslated region (3'UTR) contains a highly conserved region, which is sufficient for the perinuclear localization of a reporter mRNA. This region was shown to specifically bind protein(s) by band shift analyses. UV-cross-linking studies suggest these proteins are 46- and 35-kDa in mass. Here, we have used this sequence as 'bait' to isolate RNA binding proteins using the yeast three-hybrid method. This technique relies on a functional assay detecting bona fide RNA-protein interaction in vivo. Three cDNA isolates, HAX-1, eEF-1gamma and hRIP, code for proteins of a size consistent with in vitro cross-linking studies. In all cases, recombinant proteins were capable of binding RNA in vitro. Although hRIP is thought to be a general mRNA binding protein, this represents an unreported activity for eEF-1gamma and HAX-1. Moreover, HAX-1 binding appears to be specific to vimentin's 3'UTR. Both in vivo synthesized eEF-1gamma and HAX-1 proteins were 'pulled out' of HeLa whole cell extracts by binding to a RNA affinity column comprised of vimentin's 3'UTR. Moreover, size-fractionation of extracts results in the separation of large complexes containing either eEF-1gamma or HAX-1. Thus, in addition to their known functions, both eEF-1gamma and HAX-1 are RNA binding proteins, which suggests new roles in mRNA translation and/or perinuclear localization."xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkf656"xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/author | "Zehner Z.E."xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/author | "Allen C."xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/author | "Holmes W.M."xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/author | "Brule H."xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/author | "Al-Maghrebi M."xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/author | "Padkina M."xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/pages | "5017-5028"xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/title | "The 3' untranslated region of human vimentin mRNA interacts with protein complexes containing eEF-1gamma and HAX-1."xsd:string |
| http://purl.uniprot.org/citations/12466525 | http://purl.uniprot.org/core/volume | "30"xsd:string |
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