| http://purl.uniprot.org/citations/12475973 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12475973 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12475973 | http://www.w3.org/2000/01/rdf-schema#comment | "RNA polymerase II (pol II) is subject to an early elongation delay induced by negative factors Spt5/Spt4 and NELF, which is overcome by the positive factor P-TEFb (Cdk9/cyclin T), a protein kinase that phosphorylates the pol II C-terminal domain (CTD) and the transcription elongation factor Spt5. Although the rationale for this arrest and restart is unclear, recent studies suggest a connection to mRNA capping, which is coupled to transcription elongation via physical and functional interactions between the cap-forming enzymes, the CTD-PO(4), and Spt5. Here we identify a novel interaction between fission yeast RNA triphosphatase Pct1, the enzyme that initiates cap formation, and Schizosaccharomyces pombe Cdk9. The C-terminal segment of SpCdk9 comprises a Pct1-binding domain distinct from the N-terminal Cdk domain. We show that the Cdk domain interacts with S. pombe Pch1, a homolog of cyclin T, and that the purified recombinant SpCdk9/Pch1 heterodimer can phosphorylate both the pol II CTD and the C-terminal domain of S. pombe Spt5. We provide genetic evidence that SpCdk9 and Pch1 are functional orthologs of the Saccharomyces cerevisiae CTD kinase Bur1/Bur2, a putative yeast P-TEFb. Mutations of the kinase active site and the regulatory T-loop of SpCdk9 abolish its activity in vivo. Deleting the C-terminal domain of SpCdk9 causes a severe growth defect. We suggest a model whereby Spt5-induced arrest of early elongation ensures a temporal window for recruitment of the capping enzymes, which in turn attract Cdk9 to alleviate the arrest. This elongation checkpoint may avoid wasteful rounds of transcription of uncapped pre-mRNAs."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m211713200"xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m211713200"xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/author | "Shuman S."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/author | "Shuman S."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/author | "Pei Y."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/author | "Pei Y."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/author | "Schwer B."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/author | "Schwer B."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/pages | "7180-7188"xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/pages | "7180-7188"xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/title | "Interactions between fission yeast Cdk9, its cyclin partner Pch1, and mRNA capping enzyme Pct1 suggest an elongation checkpoint for mRNA quality control."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/title | "Interactions between fission yeast Cdk9, its cyclin partner Pch1, and mRNA capping enzyme Pct1 suggest an elongation checkpoint for mRNA quality control."xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12475973 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12475973 |
| http://purl.uniprot.org/citations/12475973 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12475973 |
| http://purl.uniprot.org/citations/12475973 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12475973 |
| http://purl.uniprot.org/citations/12475973 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12475973 |