http://purl.uniprot.org/citations/12485163 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/12485163 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundThe LR domain of marsupial chmadrin is defined by its C-terminal amino acid sequence, which contains several pairs of leucine (L) and arginine (R) residues. The LR domain of chmadrin causes a significant compaction of chromatin over the entire length of chromosomes when it is overproduced. The possible human homologue of chmadrin, Ki-67 antigen (pKi-67), also has a stretch of LR pairs, but with no obvious overall similarity, at its C-terminus.ResultsThe LR domain of human pKi-67 also induced chromatin compaction, both in human and marsupial cells. A yeast two-hybrid assay and an in vitro binding assay demonstrated that the human LR domain binds to heterochromatin protein 1 (HP1), a well-characterized molecule as a mediator of heterochromatin formation. In fixed cells stained with specific antibodies, the pKi-67 was found to be co-localized partially with HP1 at foci on chromosomes in an early G1 phase. Time-lapse observation in living cells co-expressing the fluorescently tagged proteins showed that the LR domain formed foci on chromosomes over a limited period of the cell cycle from the telophase to early G1 phase and that HP1 subsequently accumulated at these foci of the LR domain.ConclusionsMarsupial chmadrin and human pKi-67 induce chromatin compaction across species, possibly via the interaction of its LR domain with HP1."xsd:string |
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http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/author | "Hayakawa T."xsd:string |
http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/author | "Haraguchi T."xsd:string |
http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/author | "Takagi M."xsd:string |
http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/author | "Yoneda Y."xsd:string |
http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/author | "Hiraoka Y."xsd:string |
http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/author | "Kametaka A."xsd:string |
http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/date | "2002"xsd:gYear |
http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/name | "Genes Cells"xsd:string |
http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/pages | "1231-1242"xsd:string |
http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/title | "Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins."xsd:string |
http://purl.uniprot.org/citations/12485163 | http://purl.uniprot.org/core/volume | "7"xsd:string |
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