| http://purl.uniprot.org/citations/12499201 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12499201 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12499201 | http://www.w3.org/2000/01/rdf-schema#comment | "Clinical isolate Acinetobacter baumannii CLA-1 was resistant to a series of antibiotic molecules, including carbapenems. Cloning and expression of the beta-lactamase gene content of this isolate in Escherichia coli DH10B identified a chromosome-encoded oxacillinase, OXA-40, that differed by one or two amino acid changes from OXA-24, -25, and -26 and an AmpC-type cephalosporinase. The OXA-40 beta-lactamase had a mainly narrow-spectrum hydrolytic profile, but it included ceftazidime and imipenem. Its activity was resistant to inhibition by clavulanic acid, tazobactam, sulbactam, and, like most of the other carbapenem-hydrolyzing oxacillinases, NaCl. OXA-40 had an FGN triad replacing a YGN motif at class D beta-lactamase (DBL) positions 144 to 146. Site-directed DNA mutagenesis leading to a Phe-to-Tyr change at DBL position 144 in OXA-40 gave a mutant enzyme with increased hydrolytic activity against most beta-lactams, including imipenem. Conversely, with a gene encoding the narrow-spectrum oxacillinase OXA-1 as the template, a nucleotide substitution leading to a Tyr-to-Phe change in the YGN motif of OXA-1 gave a mutant enzyme with decreased hydrolytic activity without an increase in carbapenem-hydrolyzing activity. Thus, the Phe residue in the FGN motif was not associated with carbapenem-hydrolyzing activity by itself but instead was associated with weak overall hydrolytic activity. Finally, this Phe residue in OXA-40 explained resistance to inhibition by NaCl whereas a Tyr residue in motif YGN was related to susceptibility to NaCl."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.org/dc/terms/identifier | "doi:10.1128/AAC.47.1.268-273.2003"xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.org/dc/terms/identifier | "doi:10.1128/aac.47.1.268-273.2003"xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/author | "Nordmann P."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/author | "Nordmann P."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/author | "Poirel L."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/author | "Poirel L."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/author | "Aubert D."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/author | "Aubert D."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/author | "Heritier C."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/author | "Heritier C."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/name | "Antimicrob. Agents Chemother."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/name | "Antimicrob Agents Chemother"xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/pages | "268-273"xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/pages | "268-273"xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/title | "Genetic and functional analysis of the chromosome-encoded carbapenem-hydrolyzing oxacillinase OXA-40 of Acinetobacter baumannii."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/title | "Genetic and functional analysis of the chromosome-encoded carbapenem-hydrolyzing oxacillinase OXA-40 of Acinetobacter baumannii."xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/12499201 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12499201 |
| http://purl.uniprot.org/citations/12499201 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12499201 |