| http://purl.uniprot.org/citations/12499539 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12499539 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12499539 | http://www.w3.org/2000/01/rdf-schema#comment | "The three-dimensional structure of a Candida cylindracea cholesterol esterase (ChE) homodimer (534 x 2 amino acids) in complex with a ligand of proposed formula C(23)H(48)O(2) has been determined at 1.4 A resolution in space group P1 using synchrotron low-temperature data. The structure refined to R = 0.136 and R(free) = 0.169 and has revealed new stereochemical details in addition to those detected for the apo- and holo-forms at 1.9 and 2.0 A resolution, respectively [Ghosh et al. (1995), Structure, 3, 279-288]. The cholesterol esterase structure is a dimer with four spatially separated interfacial contact areas and two symmetry-related pairs of openings to an internal intradimer cavity. Hydrophobic active-site gorges in each subunit face each other across a central interfacial cavity. The ChE subunits have carbohydrate chains attached to their Asn314 and Asn351 residues, with two ordered N-acetyl-D-glucosoamine moieties visible at each site. The side chains of 14 residues have two alternative conformations with occupancy values of 0.5 +/-0.2. For each subunit the electron density in the enzyme active-site gorge is well modeled by a C(23)-chain fatty acid."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.org/dc/terms/identifier | "doi:10.1107/S0907444902018851"xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444902018851"xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/author | "Addlagatta A."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/author | "Addlagatta A."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/author | "Wawrzak Z."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/author | "Wawrzak Z."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/author | "Duax W."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/author | "Duax W."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/author | "Pletnev V."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/author | "Pletnev V."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/name | "Acta Crystallogr D Biol Crystallogr"xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/pages | "50-56"xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/pages | "50-56"xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/title | "Three-dimensional structure of homodimeric cholesterol esterase-ligand complex at 1.4 A resolution."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/title | "Three-dimensional structure of homodimeric cholesterol esterase-ligand complex at 1.4 A resolution."xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/volume | "59"xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://purl.uniprot.org/core/volume | "59"xsd:string |
| http://purl.uniprot.org/citations/12499539 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12499539 |
| http://purl.uniprot.org/citations/12499539 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12499539 |