| http://purl.uniprot.org/citations/12507481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12507481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12507481 | http://www.w3.org/2000/01/rdf-schema#comment | "The PhoP/PhoQ two-component regulatory system governs the adaptation to low Mg(2+) environments and virulence in several Gram-negative species. During growth in low Mg(2+), the sensor PhoQ modifies the activity of the response regulator PhoP promoting gene transcription, whereas growth in high Mg(2+) represses transcription of PhoP-activated genes. The PhoQ protein harbors a periplasmic domain of 146 amino acid residues that binds Mg(2+) in vitro and is required for Mg(2+)-mediated repression in vivo. Here, we identify periplasmic mutants of the Salmonella PhoQ protein that allow transcription of PhoP-activated genes even under high Mg(2+) concentrations. When expressed in a strain harboring a PhoP variant that is phosphorylated from acetyl phosphate, some of the mutants failed to repress PhoP-promoted transcription in high Mg(2+), whereas others displayed a wild-type ability to do so. Mutant PhoQ proteins that allowed expression of PhoP-activated genes in high Mg(2+) displayed a pattern of iron-mediated cleavage in vitro that was different from that displayed by wild-type PhoQ, indicative of altered Mg(2+) binding. A PhoQ protein with the conserved histidine residue (H277) substituted by alanine could not promote transcription of PhoP-activated genes in low Mg(2+) but could turn off expression in response to high Mg(2+). Our studies demonstrate that residues G93, W97, H120 and T156 are required for a wild-type response to Mg(2+), and suggest that Mg(2+) binding to the periplasmic domain regulates several activities in the PhoQ protein."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0022-2836(02)01268-8"xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0022-2836(02)01268-8"xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/author | "Groisman E.A."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/author | "Groisman E.A."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/author | "Chamnongpol S."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/author | "Chamnongpol S."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/author | "Cromie M."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/author | "Cromie M."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/pages | "795-807"xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/pages | "795-807"xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/title | "Mg2+ sensing by the Mg2+ sensor PhoQ of Salmonella enterica."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/title | "Mg2+ sensing by the Mg2+ sensor PhoQ of Salmonella enterica."xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/volume | "325"xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://purl.uniprot.org/core/volume | "325"xsd:string |
| http://purl.uniprot.org/citations/12507481 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12507481 |
| http://purl.uniprot.org/citations/12507481 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12507481 |
| http://purl.uniprot.org/citations/12507481 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12507481 |
| http://purl.uniprot.org/citations/12507481 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12507481 |