| http://purl.uniprot.org/citations/12525704 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12525704 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12525704 | http://www.w3.org/2000/01/rdf-schema#comment | "Oocyte meiosis and early mitotic divisions in developing embryos rely on the timely production of cell cycle regulators and their clearance via proteasomal degradation. Ret Finger Protein-Like 4 (Rfpl4), encoding a RING finger-like protein with a B30.2 domain, was discovered during an in silico search for germ cell-specific genes. To study the expression and functions of RFPL4 protein, we performed immunolocalizations and used yeast two-hybrid and other protein-protein interaction assays. Immunohistochemistry and immunofluorescence showed that RFPL4 accumulates in all growing oocytes and quickly disappears during early embryonic cleavage. We used a yeast two-hybrid model to demonstrate that RFPL4 interacts with the E2 ubiquitin-conjugating enzyme HR6A, proteasome subunit beta type 1, ubiquitin B, as well as a degradation target protein, cyclin B1. Coimmunoprecipitation analyses of in vitro translated proteins and extracts of transiently cotransfected Chinese hamster ovary (CHO)-K1 cells confirmed these findings. We conclude that, like many RING-finger containing proteins, RFPL4 is an E3 ubiquitin ligase. The specificity of its expression and these interactions suggest that RFPL4 targets cyclin B1 for proteasomal degradation, a key aspect of oocyte cell cycle control during meiosis and the crucial oocyte-to-embryo transition to mitosis."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0234474100"xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0234474100"xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/author | "Yan W."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/author | "Yan W."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/author | "Matzuk M.M."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/author | "Matzuk M.M."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/author | "Burns K.H."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/author | "Burns K.H."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/author | "Suzumori N."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/author | "Suzumori N."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/pages | "550-555"xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/pages | "550-555"xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/title | "RFPL4 interacts with oocyte proteins of the ubiquitin-proteasome degradation pathway."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/title | "RFPL4 interacts with oocyte proteins of the ubiquitin-proteasome degradation pathway."xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/volume | "100"xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://purl.uniprot.org/core/volume | "100"xsd:string |
| http://purl.uniprot.org/citations/12525704 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12525704 |
| http://purl.uniprot.org/citations/12525704 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12525704 |