| http://purl.uniprot.org/citations/12547713 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12547713 | http://www.w3.org/2000/01/rdf-schema#comment | "Intracytoplasmic free calcium ions (Ca++) are maintained at a very low concentration in mammalian tissue by extruding Ca++ from the cytoplasm against a steep extracellular Ca++ concentration gradient, mainly through the activity of plasma membrane Ca++ pump-ATPase. The present study aimed to elucidate how endothelin-1 (ET-1) affects the morphology of sinusoidal endothelial fenestrae and ultrastructural distribution of plasma membrane ATPases and intracytoplasmic free Ca++ in isolated rat hepatic sinusoidal endothelial cells. Sinusoidal endothelial fenestrae were observed by scanning electron microscope. Ando's electron cytochemical method was used for ultrastructural localization of Ca++-Mg++-ATPase activity, electron immunogold postembedding method for Ca++ pump-ATPase immunoactivity, and antimonate method for intracytoplasmic free Ca++. Addition of ET-1 to sinusoidal endothelial cells significantly decreased Ca++-Mg++-ATPase activity and Ca++ pump-ATPase expression and increased intracytoplasmic free Ca++ concentration, concomitant with a decrease in diameter of sinusoidal endothelial fenestrae. Co-treatment with Bosentan abolished the actions of ET-1. These results suggest that ET-1 suppresses Ca++-Mg++-ATPase activity and Ca++ pump-ATPase expression on the plasma membrane of sinusoidal endothelial fenestrae, thereby attenuating the extrusion of intracytoplasmic free Ca++ into the extracellular space, leading to an increased concentration of intracytoplasmic free calcium ions and contraction of sinusoidal endothelial fenestrae."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0002-9440(10)63849-7"xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/author | "Nomura M."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/author | "Yoshimura K."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/author | "Fujimaki K."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/author | "Oda M."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/author | "Kamegaya Y."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/author | "Ishii H."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/author | "Ogi M."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/author | "Tsukada N."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/author | "Yokomori H."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/name | "Am J Pathol"xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/pages | "557-566"xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/title | "Endothelin-1 suppresses plasma membrane Ca++-ATPase, concomitant with contraction of hepatic sinusoidal endothelial fenestrae."xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://purl.uniprot.org/core/volume | "162"xsd:string |
| http://purl.uniprot.org/citations/12547713 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12547713 |
| http://purl.uniprot.org/citations/12547713 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12547713 |
| http://purl.uniprot.org/uniprot/#_A6J756-mappedCitation-12547713 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12547713 |
| http://purl.uniprot.org/uniprot/#_P22388-mappedCitation-12547713 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12547713 |
| http://purl.uniprot.org/uniprot/#_Q9JLX8-mappedCitation-12547713 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12547713 |
| http://purl.uniprot.org/uniprot/Q9JLX8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12547713 |
| http://purl.uniprot.org/uniprot/A6J756 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12547713 |
| http://purl.uniprot.org/uniprot/P22388 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12547713 |