| http://purl.uniprot.org/citations/12549087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12549087 | http://www.w3.org/2000/01/rdf-schema#comment | "The envelope glycoprotein gD gene of pseudorabies virus Ea strain was cloned via PCR technique. Sequence analysis displayed 98% nucleotide sequence homology and 97% deduced amino acid sequence homology between our cloned gD gene and PRV Rice strain gD gene. The recombinant transfer plasmid pSX35A-gD was obtained by inserting D gene into the baculovirus transfer vector pSX35A with whole-phase promoter cassette, then transfected insect cell Hi5 with linearized AcMNPV-OCC-virus DNA, and formed recombinant baculoviruses AcMNPV-OCC(+)-gD by homologous recombination in insect cell. Recombinant baculoviruses infected insect cell Hi5 after being purified by plaque assay. Both SDS-PAGE and Western-blotting showed glycoprotein gD with a molecular weight of about 47 kD was expressed specifically, product was about 6.2% of total cellular protein, and expressed gD was of immunogenicity."xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/author | "Chen X."xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/author | "Chen Q."xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/author | "Chen H."xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/author | "Hong W."xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/author | "Wang X."xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/author | "Yang L."xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/author | "Long Q."xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/date | "2001"xsd:gYear |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/name | "Wei Sheng Wu Xue Bao"xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/pages | "329-333"xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/title | "[Cloning and expression of the envelope glycoprotein gD gene of pseudorabies virus EA strain]."xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://purl.uniprot.org/core/volume | "41"xsd:string |
| http://purl.uniprot.org/citations/12549087 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12549087 |
| http://purl.uniprot.org/citations/12549087 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12549087 |
| http://purl.uniprot.org/uniprot/#_A0A0P0VUW7-mappedCitation-12549087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12549087 |
| http://purl.uniprot.org/uniprot/#_Q10PQ9-mappedCitation-12549087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12549087 |
| http://purl.uniprot.org/uniprot/A0A0P0VUW7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12549087 |
| http://purl.uniprot.org/uniprot/Q10PQ9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12549087 |