| http://purl.uniprot.org/citations/12551919 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12551919 | http://www.w3.org/2000/01/rdf-schema#comment | "The current study examines the contribution of mitochondria-derived reactive oxygen species (ROS) in tert-butyl-hydroperoxide (TBH)-induced apoptotic signaling using clones of undifferentiated pheochromocytoma (PC-12) cells that stably overexpress the human mitochondrial or cytoplasmic forms of superoxide dismutase (SOD) (viz. Mn-SOD or CuZn-SOD, respectively). Exposure of wild type cells to TBH caused an early generation of ROS (30 min) that resulted in cell apoptosis at 24 h. These responses were attenuated with N-acetylcysteine pretreatment; however, N-acetylcysteine was ineffective in cytoprotection when added after TBH-induced ROS formation. Stable overexpression of SOD isoforms caused a 2- and 3.5-fold elevation in CuZn-SOD and Mn-SOD activities in the cytoplasm and mitochondria, respectively, and 3-fold increases in cellular GSH content. Accordingly, the stable overexpression of Mn-SOD attenuated TBH-induced mitochondrial ROS generation and cell apoptosis. Whereas transient Mn-SOD expression similarly prevented PC-12 apoptosis, this was associated with increases in SOD activity but not GSH, indicating that cytoprotection by Mn-SOD overexpression is related to mitochondrial ROS elimination and not due to increases in cellular GSH content per se. Stable or transient CuZn-SOD overexpression exacerbated cell apoptosis in conjunction with accelerated caspase-3 activation, regardless of cell GSH levels. Collectively, our results support a role for mitochondrial ROS in TBH-induced PC-12 apoptosis that is attenuated by Mn-SOD overexpression and is independent of cellular GSH levels per se."xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m208670200"xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/author | "Harrison L."xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/author | "Aw T.Y."xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/author | "Ekshyyan O.Y."xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/author | "Fuseler J."xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/author | "Pias E.K."xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/author | "Rhoads C.A."xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/pages | "13294-13301"xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/title | "Differential effects of superoxide dismutase isoform expression on hydroperoxide-induced apoptosis in PC-12 cells."xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12551919 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12551919 |
| http://purl.uniprot.org/citations/12551919 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12551919 |
| http://purl.uniprot.org/uniprot/P04179#attribution-BF744C32933F3331B57997ABCDAE372E | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12551919 |
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