| http://purl.uniprot.org/citations/12562993 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12562993 | http://www.w3.org/2000/01/rdf-schema#comment | "In pancreatic beta-cells, voltage-dependent K(+) (Kv) channels repolarise glucose-stimulated action potentials. Kv channels are therefore negative regulators of Ca(2+) entry and insulin secretion. We have recently demonstrated that Kv2.1 mediates the majority of beta-cell voltage-dependent outward K(+) current and now investigate the function of native beta-cell Kv2.1 channels at near-physiological temperatures (32-35 degrees C). While beta-cell voltage-dependent outward K(+) currents inactivated little at room temperature, both fast-inactivation (111.5 +/- 14.3 ms) and slow-inactivation (1.21 +/-0.12 s) was observed at 32-35 degrees C. Kv2.1 mediates the fast-inactivating current observed at 32-35 degrees C, since it could be selectively ablated by expression of a dominant-negative Kv2.1 construct (Kv2.1N). The surprising ability of Kv2.1N to selectively remove the fast-inactivating component, together with its sensitivity to tetraethylammonium (TEA), demonstrate that this component is not mediated by the classically fast-inactivating and TEA-resistant channels such as Kv1.4 and 4.2. Increasing the intracellular redox state by elevating the cytosolic NADPH/NADP(+) ratio from 1/10 to 10/1 increased the rates of both fast- and slow-inactivation. In addition, increasing the intracellular redox state also increased the relative contribution of the fast-inactivation component from 38.8 +/- 2.1 % to 55.9 +/-1.8 %. The present study suggests that, in beta-cells, Kv2.1 channels mediate a fast-inactivating K(+) current at physiological temperatures and may be regulated by the metabolic generation of NADPH."xsd:string |
| http://purl.uniprot.org/citations/12562993 | http://purl.org/dc/terms/identifier | "doi:10.1113/jphysiol.2002.035709"xsd:string |
| http://purl.uniprot.org/citations/12562993 | http://purl.uniprot.org/core/author | "Wheeler M.B."xsd:string |
| http://purl.uniprot.org/citations/12562993 | http://purl.uniprot.org/core/author | "MacDonald P.E."xsd:string |
| http://purl.uniprot.org/citations/12562993 | http://purl.uniprot.org/core/author | "Salapatek A.M."xsd:string |
| http://purl.uniprot.org/citations/12562993 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12562993 | http://purl.uniprot.org/core/name | "J Physiol"xsd:string |
| http://purl.uniprot.org/citations/12562993 | http://purl.uniprot.org/core/pages | "647-653"xsd:string |
| http://purl.uniprot.org/citations/12562993 | http://purl.uniprot.org/core/title | "Temperature and redox state dependence of native Kv2.1 currents in rat pancreatic beta-cells."xsd:string |
| http://purl.uniprot.org/citations/12562993 | http://purl.uniprot.org/core/volume | "546"xsd:string |
| http://purl.uniprot.org/citations/12562993 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12562993 |
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| http://purl.uniprot.org/uniprot/P15387 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12562993 |
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