| http://purl.uniprot.org/citations/12581638 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12581638 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12581638 | http://www.w3.org/2000/01/rdf-schema#comment | "ModE is a bacterial transcriptional regulator that orchestrates many aspects of molybdenum metabolism by binding to specific DNA sequences in a molybdate-dependent fashion. We present the crystal structure of Escherichia coli ModE in complex with molybdate, which was determined at 2.75A from a merohedrally twinned crystal (twin fraction approximately 0.30) with space group P4(3). We now have structures of ModE in both its "switched on" (ligand-bound) and "switched off" (apo) states. Comparison with the apo structure shows that ligand binding leads to extensive conformational changes not only in the molybdate-binding domain, but also in the DNA-binding domain. The most obvious difference is the loss of the pronounced asymmetry between the two chains of the ModE dimer, which had been a characteristic property of the apo structure. Another major change concerns the relative orientation of the two DNA-interacting winged helix-turn-helix motifs. Manual docking of an idealized DNA structure suggests that this conformational change should improve DNA binding of the activated molybdate-bound ModE."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0022-2836(02)01358-x"xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0022-2836(02)01358-x"xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/author | "Hunter W.N."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/author | "Hunter W.N."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/author | "Schuttelkopf A.W."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/author | "Schuttelkopf A.W."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/author | "Boxer D.H."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/author | "Boxer D.H."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/pages | "761-767"xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/pages | "761-767"xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/title | "Crystal structure of activated ModE reveals conformational changes involving both oxyanion and DNA-binding domains."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/title | "Crystal structure of activated ModE reveals conformational changes involving both oxyanion and DNA-binding domains."xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/volume | "326"xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://purl.uniprot.org/core/volume | "326"xsd:string |
| http://purl.uniprot.org/citations/12581638 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12581638 |
| http://purl.uniprot.org/citations/12581638 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12581638 |
| http://purl.uniprot.org/citations/12581638 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12581638 |
| http://purl.uniprot.org/citations/12581638 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12581638 |