| http://purl.uniprot.org/citations/12595532 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12595532 | http://www.w3.org/2000/01/rdf-schema#comment | "Chronic hepatitis C virus (HCV) infection frequently leads to liver cancer. To determine the viral factor(s) potentially involved in viral persistence, we focused our work on NS2, a viral protein of unknown function. To assign a role for NS2, we searched for cellular proteins that interact with NS2. Performing a two-hybrid screen on a human liver cDNA library, we found that NS2 interacted with the liver-specific pro-apoptotic CIDE-B protein. Binding specificity of NS2 for CIDE-B was confirmed by cell-free assays associated with colocalization studies and coprecipitation experiments on human endogenous CIDE-B. CIDE-B, a member of the novel CIDE family of apoptosis-inducing factors, has been reported to show strong cell death-inducing activity in its C-terminal domain. We show that this CIDE-B killing domain is involved in the NS2 interaction. NS2 binding was sufficient to inhibit CIDE-B-induced apoptosis because an NS2 deletion mutant unable to interact with CIDE-B in vitro lost its capacity to interfere with CIDE-B cell death activity. Although it has been reported that CIDE-B-induced apoptosis is characterized by mitochondrial localization, the precise apoptotic mechanism remained unknown. Here, we show that CIDE-B induced cell death in a caspase-dependent manner through cytochrome c release from mitochondria. Furthermore, we found that NS2 counteracted the cytochrome c release induced by CIDE-B. In vivo, the CIDE-B protein level was extremely low in adenovirus-infected transgenic mice expressing the HCV polyprotein compared with that in wild-type mice. We suggest that NS2 interferes with the CIDE-B-induced death pathway and participates in HCV strategies to subvert host cell defense."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m209732200"xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/author | "Gripon P."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/author | "Guguen-Guillouzo C."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/author | "Le Seyec J."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/author | "Franck N."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/author | "Lerat H."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/author | "Hibner U."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/author | "Gilot D."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/author | "Cannie I."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/author | "Erdtmann L."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/pages | "18256-18264"xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/title | "The hepatitis C virus NS2 protein is an inhibitor of CIDE-B-induced apoptosis."xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12595532 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12595532 |
| http://purl.uniprot.org/citations/12595532 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12595532 |
| http://purl.uniprot.org/uniprot/Q9UHD4#attribution-71EAE5B584DDD0E84D25E59AE4F1114B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12595532 |
| http://purl.uniprot.org/uniprot/Q9UHD4#attribution-EC2C3BE3F777FD9A20A14D0440AFFF73 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12595532 |
| http://purl.uniprot.org/uniprot/#_Q9UHD4-mappedCitation-12595532 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12595532 |
| http://purl.uniprot.org/uniprot/Q9UHD4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/12595532 |