| http://purl.uniprot.org/citations/12598536 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12598536 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12598536 | http://www.w3.org/2000/01/rdf-schema#comment | "Platelet-derived growth factor (PDGF)-C is a novel member of the PDGF family that binds to PDGF alphaalpha and alphabeta receptors. The growth factor domain of PDGF-C (GFD-PDGF-C) was expressed in high yields in Escherichia coli and was purified and refolded from inclusion bodies obtaining a biologically active growth factor with dimeric structure. The GFD-PDGF-C contains 12 cysteine residues, and Ellman assay analysis indicates that it contains three intramonomeric disulfide bonds, which is in accordance with GFD-PDGF-C being a member of the cystine knot superfamily of growth factors. The recombinant GFD-PDGF-C was characterized by CD, fluorescence, NMR, and infrared spectroscopy. Together, our data indicate that GFD-PDGF-C is a highly thermostable protein that contains mostly beta-sheet secondary structure and some (6%) alpha-helix structure. The structural model of PDGF-C, obtained by homology-based molecular modeling using the structural representatives of this family of growth factors, shows that GFD-PDGF-C has a higher structural homology to the vascular endothelial growth factor than to PDGF-B. The modeled structure can give further insights into the function and specificity of this molecule."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m301728200"xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m301728200"xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Lillehaug J.R."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Lillehaug J.R."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Martinez A."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Martinez A."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Reigstad L.J."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Reigstad L.J."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Bruland O."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Bruland O."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Varhaug J.E."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Varhaug J.E."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Fluge O."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Fluge O."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Muga A."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Muga A."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Sande H.M."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/author | "Sande H.M."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12598536 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |