| http://purl.uniprot.org/citations/12603336 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12603336 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12603336 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/12603336 | http://www.w3.org/2000/01/rdf-schema#comment | "Heterologous expression of the putative open reading frame MJ0303 of Methanococcus jannaschii provided a recombinant protein catalysing the formation of the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, by condensation of 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione and 3,4-dihydroxy-2-butanone 4-phosphate. Steady state kinetic analysis at 37 degrees C and pH 7.0 indicated a catalytic rate of 11 nmol.mg-1.min-1; Km values for 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione and 3,4-dihydroxybutanone 4-phosphate were 12.5 and 52 micro m, respectively. The enzyme sediments at an apparent velocity of about 12 S. Sedimentation equilibrium analysis indicated a molecular mass around 1 MDa but was hampered by nonideal solute behaviour. Negative-stained electron micrographs showed predominantly spherical particles with a diameter of about 150 A. The data suggest that the enzyme from M. jannaschii can form capsids with icosahedral 532 symmetry consisting of 60 subunits."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1432-1033.2003.03478.x"xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1432-1033.2003.03478.x"xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/author | "Fischer M."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/author | "Fischer M."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/author | "Bacher A."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/author | "Bacher A."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/author | "Haase I."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/author | "Haase I."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/author | "Kohler P."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/author | "Kohler P."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/author | "Mortl S."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/author | "Mortl S."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/pages | "1025-1032"xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/pages | "1025-1032"xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/title | "Biosynthesis of riboflavin in archaea. 6,7-dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/title | "Biosynthesis of riboflavin in archaea. 6,7-dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii."xsd:string |
| http://purl.uniprot.org/citations/12603336 | http://purl.uniprot.org/core/volume | "270"xsd:string |