| http://purl.uniprot.org/citations/12606581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12606581 | http://www.w3.org/2000/01/rdf-schema#comment | "Microtubules are cylindrical cytoskeletal structures found in almost all eukaryotic cell types which are involved in a great variety of cellular processes. Reversible acetylation on the epsilon-amino group of alpha-tubulin Lys40 marks stabilized microtubule structures and may contribute to regulating microtubule dynamics. Yet, the enzymes catalysing this acetylation/deacetylation have remained unidentified until recently. Here we report that beta-tubulin interacts with histone deacetylase-6 (HDAC-6) in a yeast two-hybrid assay and in vitro. We find that HDAC-6 is a micro tubule-associated protein capable of deacetylating alpha-tubulin in vivo and in vitro. HDAC-6's microtubule binding and deacetylation functions both depend on the hdac domains. Overexpression of HDAC-6 in mammalian cells leads to tubulin hypoacetylation. In contrast, inhibition of HDAC-6 function by two independent mechanisms--pharmacological (HDAC inhibitors) or genetic (targeted inactivation of HDAC-6 in embryonic stem cells)--leads to hyperacetylation of tubulin and microtubules. Taken together, our data provide evidence that HDAC-6 might act as a dual deacetylase for tubulin and histones, and suggest the possibility that acetylated non-histone proteins might represent novel targets for pharmacological therapy by HDAC inhibitors."xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/cdg115"xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/author | "Li N."xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/author | "Hess D."xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/author | "Caron C."xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/author | "Khochbin S."xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/author | "Matthias P."xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/author | "Matthias G."xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/pages | "1168-1179"xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/title | "HDAC-6 interacts with and deacetylates tubulin and microtubules in vivo."xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/12606581 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12606581 |
| http://purl.uniprot.org/citations/12606581 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12606581 |
| http://purl.uniprot.org/uniprot/Q9Z2V5#attribution-951013C716EFD27922C062B26B6F67C4 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12606581 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GX25-mappedCitation-12606581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12606581 |
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| http://purl.uniprot.org/uniprot/#_B1AUA9-mappedCitation-12606581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12606581 |
| http://purl.uniprot.org/uniprot/#_Q3U4Q5-mappedCitation-12606581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12606581 |
| http://purl.uniprot.org/uniprot/#_Q3UG37-mappedCitation-12606581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12606581 |
| http://purl.uniprot.org/uniprot/#_Q8CGC3-mappedCitation-12606581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12606581 |
| http://purl.uniprot.org/uniprot/#_Q9Z2V5-mappedCitation-12606581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12606581 |